Appendage regeneration has been widely studied in many species. Compared to other animal models, Harmonia axyridis has the advantage of a short life cycle, is easily reared, has strong regeneration capacity and contains systemic RNAi, making it a model organism for research on appendage regeneration. Here, we performed transcriptome analysis, followed by gene functional assays to reveal the molecular mechanism of H. axyridis leg regenerative growth process. Signaling pathways including Decapentaplegic (Dpp), Wingless (Wg), Ds/Ft/Hippo, Notch, Egfr, and Hedgehog (Hh) were all upregulated during the leg regenerative patterning and growth. Among these, Hh and its auxiliary receptor Lrp2 were required for the proper patterning and growth of the regenerative leg. The targets of canonical Hh signaling were required for the regenerative growth which contributes to the leg length, but were not essential for the pattern formation of the regenerative leg. dpp, wg and leg developmental-related genes including rn, dac and Dll were all regulated by hh and lrp2 and may play an essential role in the regenerative patterning of the leg.

附属物再生已在许多物种中得到广泛研究。与其他动物模型相比，异色和谐虫具有生命周期短，易于饲养，具有强大的再生能力并含有系统性RNAi的优势，使其成为研究肢体再生的模型生物。在这里，我们进行了转录组分析，然后进行了基因功能分析，以揭示腋臭线虫的分子机制。腿部再生生长过程。在腿部再生模式和生长过程中，十足瘫痪（Dpp），无翅（Wg），Ds / Ft / Hippo，Notch，Egfr和Hedgehog（Hh）等信号通路均被上调。其中，Hh及其辅助受体Lrp2是再生腿正常形成和生长所必需的。规范的Hh信号转导的目标是再生生长的必需条件，它有助于腿长，但对于再生腿的模式形成并不是必需的。民进党，WG和腿部发育相关的基因，包括氡，DAC和DLL的都被调控HH和LRP2 并可能在腿部的再生形态中起重要作用。