The true phylogenetic relation among Epinephelus species is under debate. The present investigation was designed for evaluation of DNA polymorphism in some Red sea Epinephelus species using 12s rRNA and Inter Simple Sequence repeats. DNA polymorphism values were detected and evaluated within all estimated fishes. Both applied techniques revealed that E. malabaricus is closely related to E. summana. The distance value between E. chlorostigma and E. areolatus is lower than the distance value between E. chlorostigma and E. radiatus. The Serranidae evolutionary variations were evaluated comparatively with other ray-finned fishes belonging to four fish genera (Carangidae, Labridae, Mugilidae and Cichlidae) based on 12s rRNA sequence variations (obtained from NCBI). The developed DNA markers were reliably branched in the evaluated fishes. More molecular investigations using more spatial and temporal fish samples should be carried out in the future for exploring the true Epinephelus species biodiversity in the Red sea.

中文翻译：

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使用12s rRNA和简单序列重复序列评估红海Sea鱼物种的DNA多态性

石斑鱼物种之间真正的系统发育关系尚在争论中。本研究旨在使用12s rRNA和Inter Simple Sequence重复序列来评估某些红海石斑鱼物种的DNA多态性。在所有估计的鱼类中检测并评估了DNA多态性值。这两种应用的技术表明，E.带石斑鱼是密切相关的E. summana。之间的距离值E. chlorostigma和E. areolatus比之间的距离值的情况下E. chlorostigma和E.绿豆。根据12s rRNA序列变异（从NCBI获得），对Serranidae的进化变异与属于四个鱼类（Carangidae，Labridae，Muggilidae和Cichlidae）的其他鳍鳍鱼类进行了比较评估。所开发的DNA标记可靠地分支在评估的鱼类中。将来应该使用更多的时空鱼类样品进行更多的分子研究，以探索红海中真正的石斑鱼物种生物多样性。