当前位置：
X-MOL 学术
›
Soft Matter
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Synthesis and characterization of photocrosslinkable albumin-based hydrogels for biomedical applications.
Soft Matter ( IF 2.9 ) Pub Date : 2020-09-08 , DOI: 10.1039/d0sm00977f Darlin Lantigua 1 , Michelle A Nguyen 2 , Xinchen Wu 1 , Sanika Suvarnapathaki 1 , Seongjin Kwon 2 , Wendy Gavin 3 , Gulden Camci-Unal 4
Soft Matter ( IF 2.9 ) Pub Date : 2020-09-08 , DOI: 10.1039/d0sm00977f Darlin Lantigua 1 , Michelle A Nguyen 2 , Xinchen Wu 1 , Sanika Suvarnapathaki 1 , Seongjin Kwon 2 , Wendy Gavin 3 , Gulden Camci-Unal 4
Affiliation
|
Protein-based biomaterials are widely used to generate three-dimensional (3D) scaffolds for tissue regeneration as well as compact delivery systems for drugs, genes, and peptides. Specifically, albumin-based biomaterials are of particular interest for their ability to facilitate controlled delivery of drugs and other therapeutic agents. These hydrogels possess non-toxic and non-immunogenic properties that are desired in tissue engineering scaffolds. This work employs a rapid ultraviolet (UV) light induced crosslinking to fabricate bovine serum albumin (BSA) hydrogels. Using four different conditions, the BSA hydrogel properties were modulated based on the extent of glycidyl methacrylate modification in each polymer. The highly tunable mechanical behavior of the material was determined through compression tests which yielded a range of material strengths from 4.4 ± 1.5 to 122 ± 7.4 kPa. Pore size measurements also varied from 7.7 ± 1.7 to 23.5 ± 6.6 μm in the photocrosslinked gels. The physical properties of materials such as swelling and degradation were also characterized. In further evaluation, 3D scaffolds were used in cell encapsulation and in vivo implantation studies. The biocompatibility and degradability of the material demonstrated effective integration with the native tissue environment. These modifiable chemical and mechanical properties allow BSA hydrogels to be fine-tuned to a plethora of biomedical applications including regenerative medicine, in vitro cancer study models, and wound healing approaches.
中文翻译：
用于生物医学应用的可光交联的基于白蛋白的水凝胶的合成与表征。
基于蛋白质的生物材料被广泛用于生成用于组织再生的三维（3D）支架以及用于药物，基因和肽的紧凑型递送系统。具体地，基于白蛋白的生物材料由于其促进药物和其他治疗剂的受控递送的能力而特别受到关注。这些水凝胶具有组织工程支架所需的无毒和非免疫原性。这项工作采用快速紫外线（UV）诱导交联来制备牛血清白蛋白（BSA）水凝胶。使用四种不同的条件，基于每种聚合物中甲基丙烯酸缩水甘油酯改性的程度来调节BSA水凝胶的性能。通过压缩测试确定了材料的高度可调的机械性能，压缩测试产生的材料强度范围为4.4±1.5至122±7.4 kPa。在光交联凝胶中，孔径测量值也从7.7±1.7到23.5±6.6μm不等。还表征了材料的物理性质，例如溶胀和降解。在进一步评估中，将3D支架用于细胞封装和体内植入研究。该材料的生物相容性和可降解性证明与天然组织环境有效整合。这些可修改的化学和机械特性使BSA水凝胶可以微调至多种生物医学应用，包括再生医学，体外癌症研究模型和伤口愈合方法。
更新日期：2020-09-16
中文翻译：
用于生物医学应用的可光交联的基于白蛋白的水凝胶的合成与表征。
基于蛋白质的生物材料被广泛用于生成用于组织再生的三维（3D）支架以及用于药物，基因和肽的紧凑型递送系统。具体地，基于白蛋白的生物材料由于其促进药物和其他治疗剂的受控递送的能力而特别受到关注。这些水凝胶具有组织工程支架所需的无毒和非免疫原性。这项工作采用快速紫外线（UV）诱导交联来制备牛血清白蛋白（BSA）水凝胶。使用四种不同的条件，基于每种聚合物中甲基丙烯酸缩水甘油酯改性的程度来调节BSA水凝胶的性能。通过压缩测试确定了材料的高度可调的机械性能，压缩测试产生的材料强度范围为4.4±1.5至122±7.4 kPa。在光交联凝胶中，孔径测量值也从7.7±1.7到23.5±6.6μm不等。还表征了材料的物理性质，例如溶胀和降解。在进一步评估中，将3D支架用于细胞封装和体内植入研究。该材料的生物相容性和可降解性证明与天然组织环境有效整合。这些可修改的化学和机械特性使BSA水凝胶可以微调至多种生物医学应用，包括再生医学，体外癌症研究模型和伤口愈合方法。
京公网安备 11010802027423号