Detection of human papillomavirus 16 (HPV16) is essential for the diagnosis of cervix and head and neck cancer, which has prompted the development of biosensors to replace the sophisticated molecular techniques. In this paper, we employ a genosensor made with a HPV16 DNA probe (cpHPV16) immobilized on a layer-by-layer (LbL) film of chitosan and carbon nanotubes to detect a complementary sequence HPV16 oligo (ssHPV16). In addition to employing impedance spectroscopy, which has already been used in the literature, we detect ssHPV16 in buffer samples and in cancer cell line samples with UV-vis. spectroscopy. The limit of detection was 18.5 pmol L⁻¹ in impedance spectroscopy measurements and 10.9 pmol L⁻¹ in UV-vis measurements. Distinction of the various ssHPV16 concentrations and of the cancer cell line samples CasKi, SiHa, UMSCC-47, UM-SCC104 and 93-VU147T with both electrical and optical data could be made with the multidimensional projection technique referred to as interactive document mapping (IDMAP), with which we could confirm the absence of false positives by testing cell lines that did not contain ssHPV16 (JHU12, JHU13 and JHU28). The hybridization between cpHPV16 and ssHPV16 responsible for the biosensing was verified with polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS), while the concentration dependence could be modeled with a double Freundlich function. The use of optical absorbance measurements to detect ssHPV16 is promising toward a simple colorimetric detection.

中文翻译：

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使用由DNA探针制成的基因传感器在逐层基质上检测宫颈和头颈癌衍生细胞系中的HPV16

人乳头瘤病毒16（HPV16）的检测对于子宫颈癌和头颈癌的诊断至关重要，这促使生物传感器的发展取代了复杂的分子技术。在本文中，我们采用由固定在壳聚糖和碳纳米管的逐层（LbL）膜上的HPV16 DNA探针（cpHPV16）制成的基因传感器，以检测互补序列HPV16寡核苷酸（ssHPV16）。除了采用已经在文献中使用的阻抗光谱学外，我们还使用紫外可见光检测缓冲液样品和癌细胞系样品中的ssHPV16。光谱学。阻抗谱测量的检测极限为18.5 pmol L ^-1和10.9 pmol L ^-1在紫外可见测量中。可以使用称为交互式文件映射（IDMAP）的多维投影技术来区分各种ssHPV16浓度和癌细胞系样品CasKi，SiHa，UMCSC-47，UM-SCC104和93-VU147T的电学和光学数据），我们可以通过测试不含ssHPV16（JHU12，JHU13和JHU28）的细胞系来确认不存在假阳性。通过偏振调制红外反射吸收光谱法（PM-IRRAS）验证了负责生物传感的cpHPV16和ssHPV16之间的杂交，而浓度依赖性可以通过双重Freundlich函数建模。使用光学吸光度测量来检测ssHPV16有望用于简单的比色检测。