The process of in situ tumors developing into malignant tumors and exhibiting invasive behavior is extremely complicated . From a biophysical point of view, it is a phase change process affected by many factors, including cell-to-cell, cell-to-chemical material, cell-to-environment interaction, etc . In this study, we constructed spheroids based on green fluorescence metastatic breast cancer cells MDA-MB-231 to simulate malignant tumors in vitro , while constructed a three-dimensional (3D) biochip to simulate a micro-environment for the growth and invasion of spheroids. In the experiment, the 3D spheroid was implanted into the chip, and the oriented collagen fibers controlled by collagen concentration and injection rate could guide the MDA-MB-231 cells in the spheroid to undergo directional invasion. The experiment showed that the oriented fibers greatly accelerated the invasion speed of MDA-MB-231 cells compared with the traditional uniform tumor micro-enviro...

中文翻译：

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面向胶原的纤维微环境中癌症球状细胞增强侵袭的3D生物物理模型

原位肿瘤发展为恶性肿瘤并表现出侵袭性的过程极为复杂。从生物物理的角度来看，它是一个受许多因素影响的相变过程，包括细胞间，细胞间化学物质，细胞间与环境的相互作用等。在这项研究中，我们基于绿色荧光转移性乳腺癌细胞MDA-MB-231构建了球体，以在体外模拟恶性肿瘤，同时构建了三维（3D）生物芯片，以模拟用于球体生长和入侵的微环境。 。在实验中，将3D球体植入芯片中，并通过胶原蛋白浓度和注射速率控制取向的胶原纤维可以指导球体中的MDA-MB-231细胞进行定向侵袭。