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Fabrication of 3D concentric amphiphilic microparticles to form uniform nanoliter reaction volumes for amplified affinity assays.
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-09-08 , DOI: 10.1039/d0lc00698j
Ghulam Destgeer 1 , Mengxing Ouyang , Chueh-Yu Wu , Dino Di Carlo
Affiliation  

Reactions performed in uniform microscale volumes have enabled numerous applications in the analysis of rare entities (e.g. cells and molecules). Here, highly monodisperse aqueous droplets are formed by simply mixing microscale multi-material particles, consisting of concentric hydrophobic outer and hydrophilic inner layers, with oil and water. The particles are manufactured in batch using a 3D printed device to co-flow four concentric streams of polymer precursors which are polymerized with UV light. The cross-sectional shapes of the particles are altered by microfluidic nozzle design in the 3D printed device. Once a particle encapsulates an aqueous volume, each “dropicle” provides uniform compartmentalization and customizable shape-coding for each sample volume to enable multiplexing of uniform reactions in a scalable manner. We implement an enzymatically-amplified immunoassay using the dropicle system, yielding a detection limit of <1 pM with a dynamic range of at least 3 orders of magnitude. Multiplexing using two types of shape-coded particles was demonstrated without cross talk, laying a foundation for democratized single-entity assays.

中文翻译:

3D同心两亲微粒的制造以形成均匀的纳升反应体积,用于扩增的亲和力测定。

以均匀的微量体积进行的反应已使稀有实体(例如,细胞和分子)。在此,通过将由同心疏水性外层和亲水性内层组成的微米级多材料颗粒与油和水简单混合,即可形成高度单分散的水滴。使用3D打印设备分批制造四颗同心的聚合物前体物流,这些聚合物前体将通过紫外线聚合而制成。颗粒的横截面形状通过3D打印设备中的微流体喷嘴设计而改变。一旦颗粒包封了水体积,每个“液滴”就会为每个样品体积提供均匀的分隔和可定制的形状编码,从而能够以可扩展的方式复用均匀的反应。我们使用液滴系统实施酶促放大免疫分析,检测限< 1 pM,动态范围至少3个数量级。演示了使用两种类型的形状编码粒子进行多路复用而不会发生串扰的情况,这为民主化的单实体分析奠定了基础。
更新日期:2020-09-29
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