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Spontaneous droplet generation via surface wetting.
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-09-08 , DOI: 10.1039/d0lc00641f Fengyi Liu 1 , Teng Xu , Wenjing Liu , Xiaoshan Zheng , Jian Xu , Bo Ma
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-09-08 , DOI: 10.1039/d0lc00641f Fengyi Liu 1 , Teng Xu , Wenjing Liu , Xiaoshan Zheng , Jian Xu , Bo Ma
Affiliation
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A surface wetting-driven droplet generation microfluidic chip was developed, and could produce droplets spontaneously once adding a drop of oil and an aqueous sample on the chip without any power source and equipment. The chip is simply composed of three drilled holes connected by a single microchannel. The aqueous sample dropped in the middle hole could be converged and segmented into monodispersed droplets spontaneously by preloading oil in the side hole, and then flow into the other side hole through the microchannel. To address the high throughput and stability in practical applications, a siphon pump was further integrated into the microfluidic chip by simply connecting oil-filled tubing also acting as a collector. In this way, droplets can be generated spontaneously with a high uniformity (CV < 3.5%) and adjustable size (30–80 μm). Higher throughput (280 Hz) and multi-sample emulsification are achieved by parallel integration of a multi-channel structure. Based on that, the microfluidic chip was used as the droplet generator for the ddPCR to absolutely quantify S. mutans DNA. This is the first time that the feasibility of droplet generation driven only by oil wettability on hydrophobic surfaces is demonstrated. It offers great opportunity for self-sufficient and portable W/O droplet generation in biomedical samples, thus holding the potential for point-of-care testing (POCT).
中文翻译：
通过表面润湿自发产生液滴。
开发了一种表面润湿驱动的液滴产生微流控芯片，一旦在芯片上添加一滴油和水性样品，无需任何电源和设备，即可自发产生液滴。该芯片仅由通过单个微通道连接的三个钻孔组成。通过将油预装到侧孔中，可以将滴在中间孔中的含水样品自发地汇聚并分割成单分散的液滴，然后通过微通道流入另一个侧孔中。为了解决实际应用中的高通量和稳定性，只需将充油的油管也用作收集器，就可以将虹吸泵进一步集成到微流控芯片中。这样，可以高均匀度（CV <3.5％）和可调大小（30–80μm）自发地产生液滴。通过多通道结构的并行集成，可以实现更高的通量（280 Hz）和多样品乳化。在此基础上，微流控芯片被用作ddPCR的液滴生成器，以进行绝对定量变形链球菌DNA。这是首次证明仅由疏水性表面上的油润湿性驱动的液滴生成可行性。它为在生物医学样品中产生自给自足的便携式W / O小滴提供了巨大的机会，因此具有即时检验（POCT）的潜力。
更新日期：2020-09-29
中文翻译：
通过表面润湿自发产生液滴。
开发了一种表面润湿驱动的液滴产生微流控芯片，一旦在芯片上添加一滴油和水性样品，无需任何电源和设备，即可自发产生液滴。该芯片仅由通过单个微通道连接的三个钻孔组成。通过将油预装到侧孔中，可以将滴在中间孔中的含水样品自发地汇聚并分割成单分散的液滴，然后通过微通道流入另一个侧孔中。为了解决实际应用中的高通量和稳定性，只需将充油的油管也用作收集器，就可以将虹吸泵进一步集成到微流控芯片中。这样，可以高均匀度（CV <3.5％）和可调大小（30–80μm）自发地产生液滴。通过多通道结构的并行集成，可以实现更高的通量（280 Hz）和多样品乳化。在此基础上，微流控芯片被用作ddPCR的液滴生成器，以进行绝对定量变形链球菌DNA。这是首次证明仅由疏水性表面上的油润湿性驱动的液滴生成可行性。它为在生物医学样品中产生自给自足的便携式W / O小滴提供了巨大的机会，因此具有即时检验（POCT）的潜力。
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