Purpose. Bisphenol A (BPA) is found in many plastic products and is thus a common environmental endocrine disruptor. Plastic-related health problems, including allergic diseases, are attracting increasing attention. However, few experimental studies have explored the effect of BPA on allergic rhinitis (AR). We explore whether BPA was directly related to the allergic inflammation induced by ovalbumin (OVA) in AR mice. Methods. We first constructed OVA-induced mouse model, and after BPA administration, we evaluated nasal symptoms and measured the serum OVA-specific IgE levels by ELISA. Th2 and Treg-related cytokines of nasal mucosa were measured by cytometric bead array. Th2 and Treg-specific transcription factor levels were assayed by PCR. The proportions of CD3⁺CD4⁺IL-4⁺Th2 and CD4⁺Helios⁺Foxp3⁺ T cells (Tregs) in spleen tissue were determined by flow cytometry. Results. Compared to OVA-only-induced mice, BPA addition increased nasal symptoms and serum OVA-specific IgE levels. OVA and BPA coexposure significantly increased IL-4 and IL-13 protein levels compared to those after OVA exposure alone. BPA plus OVA tended to decrease the IL-10 protein levels compared to those after OVA alone. Coexposure to OVA and BPA significantly increased the GATA-3-encoding mRNA level, and decreased the levels of mRNAs encoding Foxp3 and Helios, compared to those after OVA exposure alone. BPA increased the Th2 cell proportion, and decreased that of Tregs, compared to the levels with OVA alone. Conclusion. BPA exerted negative effects by exacerbating AR allergic symptoms, increasing serum OVA-specific IgE levels, and compromising Th2 and Treg responses.

中文翻译：

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双酚 A 在卵清蛋白诱导的过敏性鼻炎小鼠模型中加剧过敏性炎症。

目的。双酚 A (BPA) 存在于许多塑料产品中，因此是一种常见的环境内分泌干扰物。与塑料相关的健康问题，包括过敏性疾病，正引起越来越多的关注。然而，很少有实验研究探讨 BPA 对过敏性鼻炎 (AR) 的影响。我们探讨 BPA 是否与 AR 小鼠中卵清蛋白 (OVA) 诱导的过敏性炎症直接相关。方法。我们首先构建了 OVA 诱导的小鼠模型，在 BPA 给药后，我们评估了鼻部症状并通过 ELISA 测量了血清 OVA 特异性 IgE 水平。鼻黏膜Th2和Treg相关细胞因子采用流式细胞仪珠阵列检测。通过 PCR 测定 Th2 和 Treg 特异性转录因子水平。^{CD3 +} CD4的比例通过流式细胞术测定脾组织中的⁺ IL-4 ⁺ Th2 和 CD4 ⁺ Helios ⁺ Foxp3 ^{+ T 细胞 (Tregs)。}结果. 与仅 OVA 诱导的小鼠相比，添加 BPA 会增加鼻部症状和血清 OVA 特异性 IgE 水平。与单独暴露 OVA 后相比，OVA 和 BPA 共暴露显着增加了 IL-4 和 IL-13 蛋白水平。与单独使用 OVA 相比，BPA 加 OVA 倾向于降低 IL-10 蛋白水平。与单独暴露于 OVA 后相比，同时暴露于 OVA 和 BPA 显着增加了编码 GATA-3 的 mRNA 水平，并降低了编码 Foxp3 和 Helios 的 mRNA 水平。与单独使用 OVA 的水平相比，BPA 增加了 Th2 细胞比例，并降低了 Tregs 的比例。结论。BPA 通过加剧 AR 过敏症状、增加血清 OVA 特异性 IgE 水平以及损害 Th2 和 Treg 反应而产生负面影响。