Aureobasidium pullulans is the most abundant and ubiquitous species within the genus and is also considered a core component of the grape juice microflora. So far, a small number of other Aureobasidium species have been reported, that in contrast to A. pullulans, appear far more constrained to specific habitats. It is unknown whether grape juice is a reservoir of novel Aureobasidium species, overlooked in the course of conventional morphological and meta-barcoding analyses. In this study, eight isolates from grape juice taxonomically classified as Aureobasidium through ITS sequencing were subjected to whole-genome phylogenetic, synteny and nucleotide identity analyses, which revealed three isolates to likely represent newly discovered Aureobasidium species. Analyses of ITS and metagenomic sequencing datasets show that these species can be present in grape juice samples from different locations and vintages. Functional annotation revealed the Aureobasidium isolates possess the genetic potential to support growth on the surface of plants and grapes. However, the loss of several genes associated with tolerance to diverse environmental stresses suggest a more constrained ecological range than A. pullulans.

中文翻译：

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比较基因组分析提出了从葡萄汁中分离出的三种新的 Aureobasidium 物种。

Aureobasidium pullulans是该属中最丰富和普遍存在的物种，也被认为是葡萄汁微生物群落的核心成分。到目前为止，已经报道了少数其他Aureobasidium物种，与A. pullulans 相比，它们似乎更受特定栖息地的限制。尚不清楚葡萄汁是否是在常规形态学和元条形码分析过程中被忽视的新型金霉属物种的储存库。在这项研究中，从葡萄汁中分离出的八株葡萄汁在分类学上被归类为Aureobasidium通过 ITS 测序进行全基因组系统发育、同线性和核苷酸同一性分析，结果显示三个分离株可能代表新发现的Aureobasidium物种。对 ITS 和宏基因组测序数据集的分析表明，这些物种可以存在于来自不同地点和年份的葡萄汁样本中。功能注释显示，Aureobasidium分离株具有支持植物和葡萄表面生长的遗传潜力。然而，与对不同环境压力的耐受性相关的几个基因的丢失表明比A. pullulans更受限制的生态范围。