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Degradation of butachlor and propanil by Pseudomonas sp. strain But2 and Acinetobacter baumannii strain DT.
FEMS Microbiology Letters ( IF 2.1 ) Pub Date : 2020-09-08 , DOI: 10.1093/femsle/fnaa151
Ha Danh Duc 1 , Nguyen Thi Dieu Thuy 2 , Huynh Thi Thanh Truc 1 , Nguyen Thi Huynh Nhu 1 , Nguyen Thi Oanh 1
Affiliation  

Herbicides have been extensively used globally, resulting in severe environmental pollution. Novel butachlor-degrading Pseudomonas sp. strain But2 isolated from soil can degrade butachlor regardless of the concentration and grows without a lag phase. Specific degradation was increased at 0.01–0.1 mM, and did not change significantly at higher concentrations. During degradation, 2-chloro-N-(2,6-diethylphenyl) acetamide, 2,6-diethylaniline, and 1,3-diethylbenzene were formed, which indicated that deamination occurred. Moreover, Pseudomonas sp. strains could tolerate propanil at up to 0.8 mM. The mixed bacterial culture of Pseudomonas sp. But2 and Acinetobacter baumannii DT (a propanil-degrading bacterial strain) showed highly effective biodegradation of both butachlor and propanil in liquid media and soil. For example, under treatment with the mixed culture, the half-lives of propanil and butachlor were 1 and 5 days, respectively, whereas those for the control were 3 and 15 days. The adjuvants present in herbicides reduced degradation in liquid media, but did not influence herbicide removal from the soil. The results showed that the mixed bacteria culture is a good candidate for the removal of butachlor and propanil from contaminated soils.

中文翻译:

假单胞菌属降解丁草胺和丙苯胺。菌株 But2 和鲍曼不动杆菌菌株 DT。

除草剂在全球范围内广泛使用,造成了严重的环境污染。新型丁草胺降解假单胞菌。无论浓度如何,从土壤中分离出的菌株 But2 都可以降解丁草胺,并且没有滞后期生长。比降解在 0.01-0.1 mM 时增加,在较高浓度下没有显着变化。降解过程中形成了2-氯-N-(2,6-二乙基苯基)乙酰胺、2,6-二乙基苯胺和1,3-二乙基苯,表明发生了脱氨基作用。此外,假单胞菌属。菌株可以耐受高达 0.8 mM 的丙苯胺。假单胞菌的混合细菌培养。But2和鲍曼不动杆菌DT(一种丙胺降解菌株)在液体介质和土壤中显示出对丁草胺和丙胺的高效生物降解。例如,在混合培养处理下,丙虫胺和丁草胺的半衰期分别为 1 天和 5 天,而对照的半衰期分别为 3 天和 15 天。除草剂中存在的助剂减少了液体介质中的降解,但不影响从土壤中去除除草剂。结果表明,混合细菌培养物是从污染土壤中去除丁草胺和丙苯胺的良好候选物。
更新日期:2020-09-26
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