White-rot fungi is capable of producing extracellular enzymes that degrade lignin structure and facilitate biofuel production from lignocellulosic biomass wastes. However, fungal monocultures are constrained by low activities of the lignin-degrading enzyme system, leading to poor treatment efficiency and a long duration, which are not advantageous for large-scale applications. To improve enzyme production and enhance lignin degradation, a novel coculture system was proposed using the white-rot fungi Phanerochaete chrysosporium and Irpex lacteus CD2. The degradation efficiency of the alkali lignin by the fungal coculture was 26.4%, which was higher than that of the fungal monocultures. It was due to the production of lignin degrading enzymes was promoted in the liquid medium. Scanning electron microscopy, Fourier transform infrared, thermogravimetric and mercury porosimeter analyses results revealed that the alkali lignin treated with the fungal coculture had the largest porosity, and the degree of destruction of the alkali lignin structure by the fungal coculture was higher than that of the fungal monocultures. Meanwhile, the nonproductive adsorption of enzymes on alkali lignin was significantly reduced by 61.0% when the biomass was treated with the fungal coculture. As a result, the nonproductive adsorption was remarkably reduced, while it significantly improved the cellulase catalysis efficiency. These results demonstrated the synergistic effects of the fungal coculture for biomass treatment and provided a new approach for increasing lignin degradation while improving enzymatic catalysis and biofuel production through fungal coculture.

白腐真菌能够产生降解木质素结构并促进木质纤维素生物质废物产生生物燃料的细胞外酶。但是，木质素降解酶系统活性低，限制了真菌的单培养，导致处理效率低，持续时间长，不利于大规模应用。为了提高酶的产生并增强木质素的降解，提出了一种使用白腐真菌的新型共培养系统。百日草 和 乳酸菌CD2。真菌共培养对木质素的降解效率为26.4％，高于真菌单培养对木质素的降解效率。这是由于在液体培养基中促进了木质素降解酶的产生。扫描电子显微镜，傅里叶变换红外光谱，热重分析和水银孔度计分析结果表明，真菌共培养处理后的碱木质素具有最大的孔隙率，且真菌共培养对碱木质素结构的破坏程度高于真菌。单一文化。同时，用真菌共培养物处理生物量后，酶在碱性木质素上的非生产性吸附显着降低了61.0％。结果，非生产性吸附显着减少，同时大大提高了纤维素酶的催化效率。这些结果证明了真菌共培养物对生物质处理的协同作用，并提供了增加木质素降解的同时通过真菌共培养物改善酶催化和生物燃料生产的新方法。