Single-stranded breaks in the DNA backbone caused by many endogenous and exogenous agents often lead to double-stranded breaks that are known causes of chromosomal instabilities leading to copious diseases. We describe a label-free detection technique using two-dimensional (2D) solid-state nanopore field-effect transistors (FETs) to sense and map site-specific nicks in the DNA backbone. We use all-atom molecular dynamics simulations coupled with electronic transport modeling to illustrate the 2D membrane device capability to sense minute structural changes of any translocating biomolecules via their in-plane electronic sheet current signatures, whereas Van der Waals analyses explain the distinct hydrophobic interactions between various DNA-nick types with graphene and MoS₂ nanopore membranes. Specifically, we describe the atypical unzipping behavior of DNA strands caused by the biomolecule sticking at nicked site in the graphene nanopore, under the influence of voltage-specific translocations.

由许多内源性和外源性因子引起的DNA骨架中的单链断裂通常会导致双链断裂，这是导致许多疾病的染色体不稳定的已知原因。我们描述了一种使用二维（2D）固态纳米孔场效应晶体管（FET）来感测和映射DNA骨架中特定于站点的缺口的无标记检测技术。我们使用全原子分子动力学模拟与电子传输模型相结合来说明2D膜装置能够通过其平面电子片电流特征感测任何易位生物分子的微小结构变化，而Van der Waals分析解释了两者之间的独特疏水相互作用石墨烯和MoS _2的各种DNA缺口类型纳米孔膜。具体来说，我们描述了在电压特异性易位的影响下，由生物分子粘附在石墨烯纳米孔有切口的部位引起的DNA链的非典型解链行为。