Abstract

Three fungal strains belonging to the class Sordariomycetes were isolated from soils collected from Gyeongsangbuk-do in Korea. They were identified as Cephalotrichum hinnuleum (UD CT 1-3-3 and KNU-19GWF1) and Thelonectria chlamydospora sp. nov. (UD ST 1-2-1). T. chlamydospora sp. nov. was morphologically identical to T. truncata, but its specific macroconidial dimensions, lower number of septations, and chlamydospore diameter render it distinct from the strains of the genus Thelonectria. The strains UD CT 1-3-3 and KNU-19GWF1 were developed flat, velvety to felty, and golden gray to brown-gray after 14 days of incubation at 25 °C on PDA. These strains were produced polyblastic conidiogenous cells and conidia were pale brown to brown, smooth, thin-walled, subglobose to ellipsoidal, arranged in chains, and the diameters of 6.7–9.0 × 3.7–5.1 μm. The strains were also confirmed by using the multi-locus genes using internal transcribed spacer (ITS) regions, partial large subunit (LSU), translation elongation factor 1α (TEF1-α), β-tubulin (TUB2), and actin (ACT) genes. This is the discovery of T. chlamydospora sp. nov. and Cephalotrichum hinnuleum, a new record from Korea.

摘要

从韩国庆尚北道收集的土壤中分离出三种属于梭菌纲的真菌菌株。它们被鉴定为Cephalotrichum hinnuleum（UD CT 1-3-3 和 KNU-19GWF1）和Thelonectria chlamydospora sp。十一月 （UD ST 1-2-1）。T. 厚垣sp. 十一月 在形态上与T. truncata相同，但其特定的大分生孢子尺寸、较低的隔膜数量和厚垣孢子直径使其与Thelonectria属的菌株不同。25°C 在 PDA 上孵育 14 天后，菌株 UD CT 1-3-3 和 KNU-19GWF1 发育为扁平、柔软至毡状、金灰色至棕灰色。这些菌株产生多芽胞分生孢子细胞，分生孢子呈淡棕色至棕色，光滑，薄壁，近球形至椭圆形，链状排列，直径为6.7-9.0×3.7-5.1μm。这些菌株还通过使用内转录间隔区 (ITS) 区域、部分大亚基 (LSU)、翻译延伸因子 1α ( TEF1-α )、β-微管蛋白 ( TUB2 ) 和肌动蛋白 ( ACT )的多位点基因进行了确认。基因。这是T. chlamydospora sp的发现。十一月 以及来自韩国的新纪录Cephalotrichum hinnuleum 。