Abstract

Skin wound infection requires carefully long-term treatment with an immense financial burden to healthcare systems worldwide. Various strategies such as drug delivery systems using polymer matrix from natural source have been used to enhance wound healing. Natural rubber latex (NRL) from Hevea brasiliensis has shown angiogenic and tissue repair properties. Gentamicin sulfate (GS) is a broad-spectrum antibiotic which inhibits the growth of a wide variety of microorganisms and, because of this, it has also been applied topically for treatment of local infections. The aim of this study was to develop a GS release system using NRL as matrix for Staphylococcus aureus and Escherichia coli infected skin ulcers treatment, without changing drug antibiotic properties. The matrix did not change the GS antimicrobial activity against S. aureus and E. coli strains. Moreover, the NRL-GS biomembrane did not exhibit hemolytic activity, being non-toxic to red blood cells. The eluates of NRL-GS biomembranes and GS solutions did not significantly reduce the survival of Caenorhabditis elegans worms for 24 h at any of the tested concentrations. Thus, these results emphasize that the NRL-GS biomembrane proved to be a promising biomaterial for future studies on the development of dressings for topical uses, inexpensive and practicable, keeping drug antibiotic properties against pathogens and to reduce the side effects.

摘要

皮肤伤口感染需要仔细的长期治疗，这给全球医疗保健系统带来了巨大的经济负担。各种策略，例如使用来自天然来源的聚合物基质的药物递送系统，已被用于促进伤口愈合。来自巴西橡胶树的天然橡胶胶乳 (NRL)已显示出血管生成和组织修复特性。硫酸庆大霉素 (GS) 是一种广谱抗生素，可抑制多种微生物的生长，因此，它也被局部应用于治疗局部感染。本研究的目的是开发一种使用 NRL 作为金黄色葡萄球菌和大肠杆菌基质的 GS 释放系统感染性皮肤溃疡治疗，不改变药物抗生素特性。基质没有改变 GS 对金黄色葡萄球菌和大肠杆菌菌株的抗菌活性。此外，NRL-GS 生物膜不表现出溶血活性，对红细胞无毒。NRL-GS 生物膜和 GS 溶液的洗脱液在任何测试浓度下均未显着降低秀丽隐杆线虫蠕虫的存活24 小时。因此，这些结果强调，NRL-GS 生物膜被证明是一种有前途的生物材料，可用于未来研究局部使用敷料的开发，价格低廉且实用，可保持药物对病原体的抗生素特性并减少副作用。