Abstract

In this study, an ideal nano-scale material, named epidermal stimulating (ES) factors-gelatin/polycaprolactone (GT/PCL) nanofiber, was fabricated using a coaxial electrospinning technique. The ES-GT/PCL nanofibers possessed a highly porous structure with qualified mechanical properties for transplantation. With ES factors stored in the core and GT/PCL in the shell, the ES factors could be protected and released in a sustained manner. After seeding L929 cell line on ES-GT/PCL nanofibers for 7 days in vitro, the proliferation of cells was nearly 1.5 folds compared to the control group. The in vivo study showed that ES-GT/PCL nanofibers can accelerate skin wound healing rate during the healing course, especially on the early stage. The epidermal and dermal thickness, as well as skin appendages and fat tissue, were the most similar to the native skin. These findings provided valuable insights into the addition of multiple bioactive factors to nanometre biomaterials, and optimising the advantages of the compositions as a promising potential dermal substitute construct.

摘要

在这项研究中，使用同轴静电纺丝技术制造了一种理想的纳米级材料，称为表皮刺激 (ES) 因子-明胶/聚己内酯 (GT/PCL) 纳米纤维。ES-GT/PCL 纳米纤维具有高度多孔结构，具有可移植的合格机械性能。ES因子存储在核心中，GT/PCL存储在壳中，可以持续保护和释放ES因子。在体外将L929细胞系接种在ES-GT/PCL纳米纤维上7天后，细胞增殖率是对照组的近1.5倍。在体内研究表明，ES-GT/PCL 纳米纤维可以在愈合过程中加快皮肤伤口愈合速度，尤其是在早期。表皮和真皮的厚度，以及皮肤附件和脂肪组织，与原始皮肤最相似。这些发现为将多种生物活性因子添加到纳米生物材料中提供了宝贵的见解，并优化了组合物作为有前途的潜在皮肤替代结构的优势。