ABSTRACT

In order to develop the in-vitro diagnostic chips with dry chemical methods that have good diffusion performance, high accuracy of test results, and good reproducibility, the synthesis conditions of carboxylated polystyrene(PS) microspheres with uniform and controllable size, and strong surface reactivity is one of the key factors. By changing the solvent type, reaction temperature, and monomer concentration, we can control the particle size of PS microspheres. The effects of solvent type and acrylic acid concentration on the carboxyl content on the microsphere surfaces were investigated. The effect of surfactant (TX100) and stirring time on diffusion of the diffusion layer were proposed. The results showed that PS microspheres with uniform particle size were prepared with ethanol at the reaction temperature of 70°C and monomer concentration of 20 wt%. The effect of the solvent on the surface carboxyl content of PS microspheres was significant. With pentanol as the solvent, the carboxyl group content on the surface of the microspheres reached 33.9mg/g. When the stirring time is 50 min, TX100 is added as asurfactant, and the prepared diffusion layer has the best effect on diffusion.

摘要

为研制扩散性能好、检测结果准确度高、重现性好的干化学法体外诊断芯片，研制尺寸均匀可控、表面反应性强的羧化聚苯乙烯（PS）微球的合成条件是关键因素之一。通过改变溶剂类型、反应温度和单体浓度，我们可以控制PS微球的粒径。研究了溶剂类型和丙烯酸浓度对微球表面羧基含量的影响。提出了表面活性剂（TX100）和搅拌时间对扩散层扩散的影响。结果表明，以乙醇为原料，反应温度为70°C，单体浓度为20 wt%，可以制备出粒径均匀的PS微球。溶剂对PS微球表面羧基含量的影响显着。以戊醇为溶剂，微球表面羧基含量达到33.9mg/g。搅拌时间为50 min时，加入TX100作为表面活性剂，制备的扩散层对扩散效果最好。