ABSTRACT Orchid mycorrhizal fungi (OMF) are essential for orchids growth. Bletilla striata (Thunb.) Rchb.f.(Orchidaceae) has high ornamental and medicinal value. Beneficial OMF isolation is crucial to improve the survival rate of B. striata tissue culture and transplanting. In this study, we isolated and identified the beneficial OMF in B. striata from the roots of sterilized wild B. striata seedlings by culturing in four different mediums. The germination states of B. striata seeds inoculated with diverse OMF were classified and calculated. Fresh and dry weight increments of B. striata seedlings inoculated with diverse OMF were recorded after 90 d of culturing on 1/2 MS medium. ITS sequences of beneficial fungi were amplified by PCR and taxonomically identified using BLAST against the GenBank nucleotide database. Ten kinds of OMF strains were isolated from B. striata and named R1 to R10. R6 significantly promoted B. striata seeds germination (p < .01). R3 and R6 significantly promoted both the fresh and dry weight increments of B. striata seedlings (p < .05). The ITS sequence of R6 was most similar to the sequence of Serendipita. R3 was identified as Schizothecium fimbriatum by 100% ITS identity. R6 and R3 were beneficial OMF in B. striata.

中文翻译：

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Bletilla striata (Thunb.) Rchb.f.(Orchidaceae) 有益兰花菌根真菌的分离鉴定

摘要兰花菌根真菌（OMF）对兰花的生长至关重要。Bletilla striata (Thunb.) Rchb.f.(Orchidaceae) 具有很高的观赏和药用价值。有益的 OMF 隔离对于提高纹状体组织培养和移植的存活率至关重要。在这项研究中，我们通过在四种不同的培养基中培养，从已消毒的野生斑纹藻幼苗的根中分离并鉴定了斑纹藻中有益的 OMF。对接种不同 OMF 的 B. striata 种子的萌发状态进行分类和计算。在 1/2 MS 培养基上培养 90 天后，记录了接种不同 OMF 的纹状体幼苗的鲜重和干重增量。有益真菌的 ITS 序列通过 PCR 扩增，并使用 BLAST 针对 GenBank 核苷酸数据库进行分类鉴定。从 B. striata 中分离出 10 种 OMF 菌株并命名为 R1 至 R10。R6 显着促进 B. striata 种子萌发 (p < .01)。R3 和 R6 显着促进了 B. striata 幼苗的鲜重和干重增量 (p < .05)。R6 的 ITS 序列与 Serendipita 的序列最相似。通过 100% 的 ITS 身份，R3 被鉴定为 Schizothecium fimbriatum。R6 和 R3 是 B. striata 中有益的 OMF。