Soybean is an important oilseed crop and primary dietary protein resource. The limited understanding of soybean oil biosynthesis has become a significant obstacle for the improvement of soybean oil production. A transcription factor ABSCISIC ACID INSENSITIVE 3 (ABI3) is known for its role in plant development and seed dormancy in many crops. The current study was aimed to functionally characterise ABI3 homologue in Glycine max L. For this purpose, the GmABI3 gene was cloned and ectopically expressed in wildtype and abi3 mutant Arabidopsis. The GmABI3 expression in the atabi3 mutant enhanced the triacylglycerol (TAG) content (7.3%) in addition to modified fatty acid composition. The GmABI3 increased eicosenoic acid (20:1) up to 6.5% in genetically complemented Arabidopsis mutant seeds, which is essential for long-chain fatty acid synthesis. The transgenic GmABI3/wildtype seeds contain 34.9% more TAG content compared with wildtype seeds. The results showed that GmABI3 is responsible for seed-specific TAG and long-chain fatty acid biosynthesis in soybean. The exposure to cold and heat stress and exogenous supply of abscisic acid and jasmonic acid altered the level of GmABI3 in treated seeds and leaves. It also concluded that GmABI3 could regulate stress tolerance in soybean, which applies to a wide variety of crops to deal with biological stresses.

大豆是重要的油籽作物和主要膳食蛋白质资源。对大豆油生物合成的有限认识已成为提高大豆油产量的重大障碍。转录因子脱落酸不敏感 3 (ABI3) 以其在许多作物的植物发育和种子休眠中的作用而闻名。当前的研究旨在对大豆中的ABI3同源物进行功能表征。为此目的，在野生型和abi3突变体拟南芥中克隆并异位表达GmABI3基因。 atabi3突变体中的GmABI3表达除了改变脂肪酸组成外还提高了三酰甘油 (TAG) 含量 (7.3%)。 GmABI3在基因互补的拟南芥突变体种子中将二十碳烯酸 (20:1) 增加高达 6.5%，这对于长链脂肪酸的合成至关重要。与野生型种子相比，转基因GmABI3 /野生型种子的TAG含量高出34.9%。结果表明， GmABI3负责大豆种子特异性 TAG 和长链脂肪酸生物合成。暴露于冷和热应激以及外源脱落酸和茉莉酸的供应改变了处理过的种子和叶子中的GmABI3水平。它还得出结论， GmABI3可以调节大豆的胁迫耐受性，这适用于多种作物应对生物胁迫。