This study reports on the effect of inoculum history, growth substrates, and yeast extract on sodium chloride tolerance of Sulfobacillus thermosulfidooxidans DSM 9293^T. The concentrations of NaCl for complete inhibition of Fe²⁺ oxidation by cells initially grown with ferrous iron sulfate, or tetrathionate, or pyrite as energy sources were 525 mM, 725 mM, and 800 mM, respectively. Noticeably, regardless of NaCl concentrations, oxygen consumption rates of S. thermosulfidooxidans with 20 mM tetrathionate were higher than with 50 mM FeSO₄. NaCl concentrations of higher than 400 mM strongly inhibited the iron respiration of S. thermosulfidooxidans. In contrast, the presence of NaCl was shown to stimulate tetrathionate oxidation. This trend was especially pronounced in NaCl-adapted cells where respiration rates at 200 mM NaCl were threefold of those in the absence of NaCl. In NaCl-adapted cultures greater respiration rates for tetrathionate were observed than in non-NaCl-adapted cultures, especially at concentrations ≥ 200 mM NaCl. At concentrations of ≤ 200 mM NaCl, cell growth and iron oxidation were enhanced with the addition of increasing concentrations of yeast extract. Thus, cell numbers in cultures with 0.05% yeast extract were ∼5 times higher than without yeast extract addition. At NaCl concentration as high as 400 mM, however, iron oxidation rates improved compared to control assays without yeast extract, but there was no clear dependence on yeast extract concentrations. The initial growth of bacteria with and without yeast extract in the presence of different NaCl concentrations was shown to impact leaching of copper from chalcopyrite. Copper dissolution was enhanced in the presence of 200 mM NaCl and absence of yeast extract, while the addition of 0.02% yeast extract was shown to promote copper solubilization in the presence of 500 mM NaCl.

这项研究报告了接种史，生长底物和酵母提取物对热硫亚砜DSM 9293 ^T对氯化钠耐受性的影响。最初以硫酸亚铁，四硫酸盐或黄铁矿为能源生长的细胞完全抑制Fe ²⁺氧化的NaCl浓度分别为525 mM，725 mM和800 mM。值得注意的是，无论NaCl浓度如何，具有20 mM四硫代酸盐的热硫脲氧化酶的耗氧率均高于具有50 mM FeSO _4的耗氧率。NaCl浓度高于400 mM时，强烈抑制了热硫氧化物氧化链球菌的铁呼吸作用。。相反，显示NaCl的存在会刺激四硫酸盐氧化。这种趋势在适应NaCl的细胞中尤为明显，在200 mM NaCl中，其呼吸速率是不存在NaCl时的呼吸速率的三倍。在适应NaCl的培养物中，与未适应NaCl的培养相比，观察到四硫酸盐的呼吸速率更高，尤其是在浓度≥200 mM NaCl的情况下。在≤200 mM NaCl的浓度下，随着酵母提取物浓度的增加，细胞的生长和铁的氧化作用得以增强。因此，含有0.05％酵母提取物的培养物中的细胞数是未添加酵母提取物时的细胞数的约5倍。但是，在NaCl浓度高达400 mM时，与没有酵母提取物的对照测定相比，铁的氧化速率有所提高，但是对酵母提取物浓度没有明显的依赖性。有和没有酵母提取物的细菌在不同NaCl浓度下的初始生长都显示出影响从黄铜矿中浸出铜的影响。在存在200 mM NaCl和没有酵母提取物的情况下，铜的溶解度得到提高，而添加0.02％酵母提取物显示出在存在500 mM NaCl的情况下促进铜的溶解。