Gold nanoclusters (AuNCs) stabilized by glutathione (GSH) have been synthesized using a simple one-pot method, which were used as a fluorescence-enhanced probe for the detection of cysteine (Cys) and GSH. The detection is based on the finding that the weak yellow fluorescence of the AuNCs, with excitation/emission maxima of 430/600 nm, can be enhanced by Cys and GSH via NCs aggregation. This method is selective for Cys and GSH. According to the fluorescence enhancement, the detection ranges of AuNCs for Cys and GSH are 2.49 µM ~ 0.80 mM and 1.99 µM ~ 0.44 mM, with the detection limit of 0.42 µM and 0.27 µM, respectively. In addition, the probe has good anti-interference performance over other common biomolecules. Importantly, the probe is successfully used for the determination of Cys in human serum samples, displaying the potential application of the probe in the detection of biological sulfhydryl molecules in actual samples.

用简单的一锅法合成了由谷胱甘肽（GSH）稳定的金纳米簇（AuNCs），它们被用作荧光增强探针，用于检测半胱氨酸（Cys）和GSH。检测基于以下发现：Cys和GSH可通过NCs聚集增强AuNCs的弱黄色荧光（激发/发射最大值为430/600 nm）。此方法对Cys和GSH具有选择性。根据荧光增强，AuNCs对Cys和GSH的检测范围为2.49 µM〜0.80 mM和1.99 µM〜0.44 mM，检出限分别为0.42 µM和0.27 µM。另外，该探针比其他常见生物分子具有良好的抗干扰性能。重要的是，该探针已成功用于人类血清样品中Cys的测定，