So far approximately 144,000 species of fungi have been named but sequences of the majority of them do not exist in the public databases. Therefore, the quality and coverage of public barcode databases is a bottleneck that hinders the study of fungi. Cortinarius is the largest genus of Agaricales with thousands of species world-wide. The most diverse subgenus in Cortinarius is Telamonia and its species have been considered one of the most taxonomically challenging in the Agaricales. Its high diversity combined with convergent, similar appearing taxa have earned it a reputation of being an impossible group to study. In this study a total of 746 specimens, including 482 type specimens representing 184 species were sequenced. Also, a significant number of old types were successfully sequenced, 105 type specimens were over 50 years old and 18 type specimens over 100 years old. Altogether, 20 epi- or neotypes are proposed for recently commonly used older names. Our study doubles the number of reliable DNA-barcodes of species of C. subgenus Telamonia in the public sequence databases. This is also the first extensive phylogenetic study of the subgenus. A majority of the sections and species are shown in a phylogenetic context for the first time. Our study shows that nomenclatural problems, even in difficult groups like C. subgenus Telamonia, can be solved and consequently identification of species based on ITS barcodes becomes an easy task even for non-experts of the genus.

迄今为止，已经命名了约144,000种真菌，但是大多数的序列在公共数据库中不存在。因此，公共条形码数据库的质量和覆盖范围是阻碍真菌研究的瓶颈。Cortinarius是Agaricales的最大属，在世界范围内有数千种。Cortinarius中最多样化的亚属是Telamonia其菌种被认为是蛤the菌中最具分类学挑战性的物种之一。它的高多样性加上类似的趋同分类单元，使其成为无法学习的群体而享有盛誉。在这项研究中，总共对746个标本进行了测序，包括代表184种的482个标本。而且，成功地测序了许多旧类型，超过50年的105个标本和超过100年的18个标本。对于最近常用的较旧名称，总共提出了20种表型或新类型。我们的研究使C物种的可靠DNA条形码数量翻了一番。特拉莫尼亚亚属在公共序列数据库中。这也是该亚属的首次广泛系统发育研究。大部分区域和物种首次在系统发育背景下显示。我们的研究表明，即使在C之类的困难群体中，也存在命名规则问题。可以解决Telamonia亚属的问题，因此即使对于非该属专家，基于ITS条形码识别物种也变得容易。