A novel carbon nanotube-collagen@hydroxyapatite (CNT-Col@HA) composite with good mechanical and biological properties was fabricated successfully by a multi in situ synthesis process, which can be used to repair or replace the damaged bone tissues. The carbon nanotube (CNT)/hydroxyapatite (HA) composite powders were firstly synthesized by the in situ chemical vapor deposition method. After the acidification of CNTs, the collagen (Col) molecules were covalently grafted onto the surface of CNTs in situ by the formation of amide linkages, obtaining Col-encapsulated CNTs powders. And then, a HA layer was deposited in situ onto the Col-encapsulated CNTs to form HA- and Col-encapsulated CNTs, consequently the ideal CNT-Col@HA composite was fabricated by the powder metallurgy method, and its mechanical and biological properties were investigated. The results showed that, the multi in situ synthesis process ensured the homogeneous dispersion of CNTs in HA matrix, and via the intermediate layer of Col, the close chemical bonding between CNT reinforcements and HA matrix was obtained, thereby the flexural strength and fracture toughness of the in situ synthesized 3 wt.% CNT-Col@HA composite were increased by approximately 74.2% and 274.6% compared with those of pure HA bulk, and better cell adhesion, spreading and proliferation were also observed on the in situ synthesized CNT-Col@HA composites. Therefore, the obtained composites in this work have great potential to be applied as implant material in clinic.

通过多原位合成工艺成功制备出一种具有良好力学和生物性能的新型碳纳米管-胶原@羟基磷灰石（CNT-Col@HA）复合材料，可用于修复或替代受损的骨组织。首次采用原位化学气相沉积法合成了碳纳米管（CNT）/羟基磷灰石（HA）复合粉末。碳纳米管酸化后，胶原蛋白（Col）分子通过形成酰胺键原位共价接枝到碳纳米管表面，得到Col包裹的碳纳米管粉末。然后，在 Col 封装的 CNT 上原位沉积 HA 层，形成 HA 和 Col 封装的 CNT，从而通过粉末冶金方法制备了理想的 CNT-Col@HA 复合材料，并对其力学和生物性能进行了研究。调查了。结果表明，多原位合成工艺保证了碳纳米管在HA基体中的均匀分散，并通过Col中间层，使CNT增强体与HA基体之间形成紧密的化学键合，从而提高了碳纳米管的弯曲强度和断裂韧性。原位合成的3 wt.% CNT-Col@HA复合材料与纯HA本体相比增加了约74.2%和274.6%，并且在原位合成的CNT-Col上还观察到更好的细胞粘附、铺展和增殖@HA复合材料。因此，本工作获得的复合材料作为临床植入材料具有巨大的应用潜力。