Mitogen Activated Protein Kinase (MAPK) cascade is a fundamental pathway in organisms for signal transduction. Though it is well characterized in various plants, there is no systematic study of this cascade in tea. In this study, 5 genes of Mitogen Activated Protein Kinase Kinase (MKK) and 16 genes of Mitogen Activated Protein Kinase (MPK) in Camellia sinensis were found through a genome-wide search taking Arabidopsis thaliana as the reference genome. Also, phylogenetic relationships along with structural analysis which includes gene structure, location as well as protein conserved motifs and domains, were systematically examined and further, predictions were validated by the results. The plant species taken for comparative study clearly displayed segmental duplication, which was a significant candidate for MAPK cascade expansion. Also, functional interaction was carried out in C. sinensis based on the orthologous genes in Arabidopsis. The expression profiles linked to various stress treatments revealed wide involvement of MAPK and MAPKK genes from Tea in response to various abiotic factors. In addition, the expression of these genes was analysed in various tissues. This study provides the targets for further comprehensive identification, functional study, and also contributed for a better understanding of the MAPK cascade regulatory network in C. sinensis.

中文翻译：

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茶树中的MAPK级联基因家族：计算机鉴定，表达谱和调控网络分析。

丝裂原活化蛋白激酶（MAPK）级联是生物体内信号转导的基本途径。尽管它在各种植物中都有很好的表征，但尚无系统研究茶中级联反应的方法。本研究以拟南芥为参考基因组，通过全基因组搜索在山茶中发现了5个丝裂素活化蛋白激酶（MKK）基因和16个丝裂素活化蛋白激酶（MPK）基因。此外，系统地研究了系统发育关系以及包括基因结构，位置以及蛋白质保守基序和结构域在内的结构分析，并进一步通过结果验证了预测。用于比较研究的植物物种清楚地显示出节段重复，这是MAPK级联扩增的重要候选者。也，基于拟南芥中的直系同源基因，在中华C中进行了功能性相互作用。与各种应激处理相关的表达谱揭示了茶对多种非生物因子的响应广泛参与了MAPK和MAPKK基因的表达。另外，分析了这些基因在各种组织中的表达。该研究为进一步全面鉴定，功能研究提供了目标，也为更好地了解中华绒螯蟹的MAPK级联调控网络做出了贡献。