Biomimetic cues from poly(lactic-co-glycolic acid)/hydroxyapatite nano-fibrous scaffolds drive osteogenic commitment in human mesenchymal stem cells in the absence of osteogenic factor supplements.,Biomaterials Science

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Biomimetic cues from poly(lactic-co-glycolic acid)/hydroxyapatite nano-fibrous scaffolds drive osteogenic commitment in human mesenchymal stem cells in the absence of osteogenic factor supplements.
Biomaterials Science ( IF 5.8 ) Pub Date : 2020-09-07 , DOI: 10.1039/d0bm00946f
Mohammad Soheilmoghaddam ₁ , Harish Padmanabhan , Justin J Cooper-White

Affiliation

Mimicking the complex hierarchical architecture of the ‘osteon’, the functional unit of cortical bone, from the bottom-up offers the possibility of generating mature bone tissue in tissue engineered bone substitutes. In this work, a modular ‘bottom-up’ approach has been developed to assemble bone niche-mimicking nanocomposite scaffolds composed of aligned electrospun nanofibers of poly(lactic-co-glycolic acid) (PLGA) encapsulating aligned rod-shape nano-sized hydroxyapatite (nHA). By encoding axial orientation of the nHA within these aligned nanocomposite fibers, significant improvements in mechanical properties, surface roughness, hydrophilicity and in vitro simulated body fluid (SBF) mineral deposition were achieved. Moreover, these hierarchical scaffolds induced robust formation of bone hydroxyapatite and osteoblastic maturation of human bone marrow-derived mesenchymal stem cells (hBMSCs) in growth media that was absent of any soluble osteogenic differentiation factors. The results of this investigation confirm that these tailored, aligned nanocomposite fibers, in the absence of media-bone inductive factors, offer the requisite biophysical and biochemical cues to hBMSCs to promote and support their differentiation into mature osteoblast cells and form early bone-like tissue in vitro.

中文翻译：

在没有成骨因子补充剂的情况下，来自聚乳酸-乙醇酸/羟基磷灰石纳米纤维支架的仿生线索可驱动人间充质干细胞的成骨作用。

从下至上模仿皮质骨功能单元“ osteon”的复杂层次结构，可以在组织工程化的骨替代物中生成成熟的骨组织。在这项工作中，一个模块化“自下而上”的方法已被开发来组装聚（乳酸-对准电纺纳米纤维构成的骨利基模仿纳米复合材料的支架共-glycolic乙酸）（PLGA）封装对齐棒形状的纳米尺寸的羟基磷灰石（nHA）。通过编码这些对齐的纳米复合纤维中nHA的轴向取向，可以显着改善机械性能，表面粗糙度，亲水性和体外性能模拟了体液（SBF）的矿物沉积。而且，这些分级支架在没有任何可溶性成骨分化因子的生长培养基中诱导骨骼羟基磷灰石的牢固形成和人骨髓来源的间充质干细胞（hBMSC）的成骨细胞成熟。这项研究的结果证实，这些定制的，对齐的纳米复合纤维在没有中骨诱导因子的情况下，为hBMSC提供了必要的生物物理和生化线索，以促进和支持其分化为成熟的成骨细胞并形成早期的骨样组织。体外。

更新日期：2020-10-13

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