Background: Immunoglobulin (Ig) G is the most commonly used therapeutic antibodies. Recently, the interest in IgA antibodies to treat respiratory infectious diseases has been increasing. The reason for the inefficient use of IgA is recombinant antibody aggregation in cell culture, affecting the longevity and productivity of cell lines. Lactate is an important metabolite that affects the cultivation of stable cell lines producing monoclonal antibodies.

Methods: In the present study, we investigated whether different combinations of succinic acid and micro-additives affect lactate production, which correlates with productivity. The effect of succinic acid substitution on productivity of cells producing IgG/IgA was analyzed using the static culture method in a six-well plate. Lactate was measured in supernatant of cell culture indirectly by using the activity of Lactate Dehydrogenase (LDH).A low lactate level was observed in cultivation medium supplemented with succinic acid or asparagine combined with some inorganic salts.

Results: The results also demonstrated the effect of component supplementation on homogeneity, longevity, and productivity of cell culture. Supplementation of succinic acid eliminated cell aggregation and improved homogeneity of stable cell lines producing IgG and, especially, IgA.

Conclusion: Overall, succinic acid supplementation to the culture medium has potential biotechnological applications in the production IgG and IgA.

背景：免疫球蛋白（Ig）G是最常用的治疗性抗体。近来，对治疗呼吸道感染性疾病的IgA抗体的兴趣不断增加。IgA使用效率低下的原因是细胞培养物中重组抗体的聚集，从而影响细胞系的寿命和生产力。乳酸是一种重要的代谢产物，会影响稳定产生单克隆抗体的细胞系的培养。

方法：在本研究中，我们调查了琥珀酸和微添加剂的不同组合是否会影响乳酸的产生，而乳酸的产生与生产率相关。使用六孔板中的静态培养方法分析了琥珀酸取代对产生IgG / IgA的细胞生产力的影响。利用乳酸脱氢酶（LDH）的活性间接测量细胞培养上清液中的乳酸。在补充有琥珀酸或天冬酰胺与一些无机盐的培养基中观察到乳酸水平较低。

结果：结果还证明了补充成分对细胞培养的均匀性，寿命和生产率的影响。补充琥珀酸消除了细胞聚集并改善了产生IgG，尤其是IgA的稳定细胞系的均质性。

结论：总的来说，向培养基中补充琥珀酸在生产IgG和IgA中具有潜在的生物技术应用。