Turn-On Assay for HIV-1 Protease Inhibitor Selection,ACS Applied Bio Materials

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Turn-On Assay for HIV-1 Protease Inhibitor Selection
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-09-07 , DOI: 10.1021/acsabm.0c00899
Yan Shi ₁ , Zhuang Li ₁ , Hongda Wang ₁

Affiliation

Virus threatens our lives and health at all times. Among them, HIV has caused millions of deaths since its discovery. Research on viral inhibitor selection has become an urgent need. A reliable turn-on assay for HIV-1 protease (HIV-1 PR) inhibitor selection has been developed. The feasibility assay of protease detection was demonstrated based on fluorescence resonance energy transfer between graphene oxide and dye-modified peptides. Besides the rapid and sensitive detection of HIV-1 PR, the enzyme kinetic parameters were also measured. Most importantly, this assay was applied in the distinction of protease inhibitor efficiency and the unknown inhibitor selection.

中文翻译：

HIV-1 蛋白酶抑制剂选择的开启检测

病毒无时无刻不在威胁着我们的生命和健康。其中，艾滋病病毒自被发现以来已造成数百万人死亡。对病毒抑制剂选择的研究已成为当务之急。已经开发了一种可靠的 HIV-1 蛋白酶 (HIV-1 PR) 抑制剂选择的开启试验。基于氧化石墨烯和染料修饰肽之间的荧光共振能量转移证明了蛋白酶检测的可行性分析。除了快速灵敏地检测 HIV-1 PR 外，还测量了酶动力学参数。最重要的是，该测定法用于区分蛋白酶抑制剂效率和未知抑制剂的选择。

更新日期：2020-11-16

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