The Application of Dual‐Layer, Mussel‐Inspired, Antifouling Polyglycerol‐Based Coatings in Ventricular Assist Devices,Advanced Materials Interfaces

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The Application of Dual‐Layer, Mussel‐Inspired, Antifouling Polyglycerol‐Based Coatings in Ventricular Assist Devices
Advanced Materials Interfaces ( IF 4.3 ) Pub Date : 2020-09-06 , DOI: 10.1002/admi.202000272
Michaël W. Kulka ₁ , Sarah Smatty ₂ , Felix Hehnen ₂ , Tim Bierewirtz ₂ , Kim Silberreis _{1,

3} , Chuanxiong Nie ₁ , Yannic Kerkhoff ₁ , Carsten Grötzinger ₄ , Sebastian Friedrich ₅ , Lars Ingemar Dahms ₆ , Jens Dernedde ₃ , Ingo Grunwald ₇ , Michael Schirner ₁ , Ulrich Kertzscher ₂ , Klaus Affeld ₂ , Rainer Haag ₁

Affiliation

Continuous‐flow ventricular assist devices (VADs) have established themselves as a lifesaving therapy option in patients with severe cardiovascular disease. Unfortunately, complications with VADs resulting from the shear‐induced formation of surface blood clots are common. In the current work, an antifouling coating based on the combination of mussel‐inspired dendritic polyglycerol (MI‐dPG) and linear polyglycerol (lPG) is tested for its cell‐repelling properties, biocompatibility, and complement activating properties. Furthermore, the adhesion and activation of blood platelets are tested under static and flow conditions. The adhesion and proliferation of two cell types are studied by means of LIVE/DEAD cell staining, and it is clearly observed that the lPG‐functionalized MI‐dPG coating prevents cell adhesion. Additionally, no cell mortality is observed on all substrates, indicating the biocompatibility of the tested coatings. All coatings show lower (or equal) complement‐activating properties than bare titanium, which is considered a highly biocompatible material. Most importantly, the lPG‐functionalized system prevents the adhesion and activation of blood platelets under static and flow conditions. Finally, a prototype VAD is successfully coated with MI‐dPG under flow conditions. In the current study, the efficient lPG‐functionalization of the MI‐dPG coating is proved to obtain cell‐ and platelet‐repelling surfaces.

中文翻译：

双层，贻贝类防污聚甘油基涂料在心室辅助装置中的应用

连续流式心室辅助设备（VAD）已成为严重心血管疾病患者的一种救生治疗选择。不幸的是，由剪切诱导的表面血凝块形成导致的VAD并发症很常见。在当前的工作中，基于贻贝启发性树突状聚甘油（MI-dPG）和线性聚甘油（lPG）的防污涂料已经过测试，具有细胞排斥特性，生物相容性和补体激活特性。此外，在静态和流动条件下测试血小板的粘附和活化。通过LIVE / DEAD细胞染色研究了两种细胞的粘附和增殖，并且清楚地观察到，由lPG功能化的MI-dPG涂层可防止细胞粘附。另外，在所有底物上均未观察到细胞死亡，表明测试涂层的生物相容性。所有涂层都显示出比裸钛低（或相等）的补体激活特性，裸钛被认为是高度生物相容性材料。最重要的是，LPG功能化的系统可防止在静态和流动条件下血小板的粘附和活化。最后，在流动条件下成功用MI-dPG涂覆VAD原型。在当前的研究中，证明了MI-dPG涂层的有效的lPG功能化可以获得排斥细胞和排斥血小板的表面。lPG功能化的系统可防止在静态和流动条件下血小板的粘附和活化。最后，在流动条件下成功用MI-dPG涂覆VAD原型。在当前的研究中，证明了MI-dPG涂层的有效的lPG功能化可以获得排斥细胞和排斥血小板的表面。lPG功能化的系统可防止在静态和流动条件下血小板的粘附和活化。最后，在流动条件下成功用MI-dPG涂覆VAD原型。在当前的研究中，证明了MI-dPG涂层的有效的lPG功能化可以获得排斥细胞和排斥血小板的表面。

更新日期：2020-11-06

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