Nanodiamond (ND)‐based nanoparticles with hyaluronic acid (HA) and chlorin e6 (Ce6) were fabricated for potential photodynamic and photothermal tumor therapy. The ND nanoparticles conjugated with HA and Ce6 (Ce6‐ND@HA) had a suitable size range about 130 nm and high zeta‐potential about −35 mV. The conjugation was confirmed by UV‐vis spectroscopy and the conjugation efficiency of Ce6 in these ND nanoparticles was quantified by fluorescence emission spectrum. HeLa and NIH/3T3 cell lines were used as model cells in order to evaluate the performance of ND, ND nanoparticles with HA (ND@HA), and Ce6‐ND@HA. The ND@HA and Ce6‐ND@HA nanoparticles had a selective uptake for HeLa cells due to the presence of HA. In addition, Ce6‐ND@HA nanoparticles had a high cell ablation effect for HeLa cells due to the temperature increase caused by ND and singlet oxygen generation caused by Ce6. These results suggest that Ce6‐ND@HA particles might have potential for targeted anti‐tumor treatment.

中文翻译：

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透明质酸和二氢卟酚e6修饰的纳米金刚石的制备，用于选择性光热和光动力肿瘤治疗

制备了具有透明质酸（HA）和二氢卟酚e6（Ce6）的纳米金刚石（ND）基纳米颗粒，用于潜在的光动力和光热肿瘤治疗。与HA和Ce6共轭的ND纳米颗粒（Ce6-ND @ HA）的合适尺寸范围约为130 nm，高ζ电位约为-35 mV。通过紫外可见光谱确认了共轭，并通过荧光发射光谱定量了这些ND纳米颗粒中Ce6的共轭效率。HeLa和NIH / 3T3细胞系用作模型细胞，以评估ND，带有HA的ND纳米颗粒（ND @ HA）和Ce6-ND @ HA的性能。由于存在HA，ND @ HA和Ce6-ND @ HA纳米颗粒对HeLa细胞具有选择性摄取。此外，由于ND引起的温度升高和Ce6引起的单线态氧的产生，Ce6-ND @ HA纳米粒子对HeLa细胞具有较高的细胞消融作用。这些结果表明，Ce6-ND @ HA颗粒可能具有靶向抗肿瘤治疗的潜力。