Overexpression of an aquaporin gene PvPIP2;9 improved biomass yield, protein content, drought tolerance and water use efficiency in switchgrass (Panicum virgatum L.),Global Change Biology Bioenergy

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Overexpression of an aquaporin gene PvPIP2;9 improved biomass yield, protein content, drought tolerance and water use efficiency in switchgrass (Panicum virgatum L.)
Global Change Biology Bioenergy ( IF 5.9 ) Pub Date : 2020-09-07 , DOI: 10.1111/gcbb.12751
Jing Zhang ₁ , Wuwu Wen ₁ , Hui Li ₁ , Qiyu Lu ₁ , Bin Xu ₁ , Bingru Huang ₂

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Switchgrass (Panicum virgatum L.) is a prime candidate for non‐grain‐based bioenergy feedstock production. Improved drought tolerance and higher water use efficiency (WUE) are important for its successful field establishment and production, especially on marginal lands. Aquaporins are key channels and regulators for water transportation and maintenance of cellular water status. In this study, the functional role of an aquaporin gene, PvPIP2;9, in switchgrass was studied. Expression of PvPIP2;9 was regulated by diurnal oscillation and osmotic stress. Constitutive over‐expressing PvPIP2;9 in switchgrass significantly improved its leaf length, plant height, aboveground biomass and biomass protein contents under both drought‐ and non‐stressed conditions, and also resulted in higher cellulose content in drought‐stressed transgenic than wild‐type plants. Under 21 days of drought treatment, transgenic plants showed less electrolyte leakage rates, but higher relative water contents, photochemical efficiencies, chlorophyll contents and WUE, indicating that PvPIP2;9 positively regulated plant drought tolerance. Moreover, expression patterns of all 14 switchgrass PIP2 subfamily genes were checked during the drought treatment, and the result showed that over‐expressing PvPIP2;9 also affected transcript levels of most other PIP2 genes. Together, this study showed that improved biomass yield, drought tolerance and higher WUE can be achieved by manipulating the expression of PvPIP2;9 and also suggested PIP2 subfamily genes were transcriptionally regulated in a coordinated manner, which can be used for potential targets for crop drought resistance breeding efforts.

中文翻译：

水通道蛋白基因PvPIP2的过表达； 9提高了柳枝（（Panicum virgatum L.）的生物量产量，蛋白质含量，耐旱性和水分利用效率

柳枝（（Panicum virgatum L.）是非谷物生物能源原料生产的主要候选产品。改善的耐旱性和更高的用水效率（WUE）对于其成功的田间建立和生产，特别是在边际土地上的成功至关重要。水通道蛋白是水运输和维持细胞水状态的关键通道和调节剂。在这项研究中，研究了水通道蛋白基因PvPIP2; 9在柳枝switch中的功能作用。的表达PvPIP2; 9通过昼夜振荡和渗透胁迫调节。组成型过表达PvPIP2; 9干旱和非胁迫条件下，柳枝switch的叶长，株高，地上生物量和生物量蛋白质含量均得到显着改善，而且干旱胁迫转基因植物中的纤维素含量也高于野生型植物。在21天的干旱处理下，转基因植物的电解质泄漏率较低，但相对水含量，光化学效率，叶绿素含量和WUE较高，表明PvPIP2; 9可以正向调节植物的抗旱性。此外，在干旱处理期间检查了全部14个柳枝switch PIP2亚家族基因的表达模式，结果表明，过表达PvPIP2; 9也影响了大多数其他PIP2的转录水平。基因。总之，这项研究表明，通过操纵PvPIP2的表达可以提高生物量产量，提高耐旱性和提高WUE ； 9并建议以协调的方式对PIP2亚家族基因进行转录调控，可将其用作潜在的作物干旱靶点抗性育种努力。

更新日期：2020-10-15

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