Analytic hierarchy process (AHP) combined with response surface methodology (RSM) was used to optimize the purification processes of the crude flavonoids of Carex meyeriana Kunth (CMK). The bioactivities of flavonoids were evaluated in terms of antioxidant and antimicrobial activities. The weighted coefficients of comprehensive scoring method on AHP included recovery, purity, and decolorization rate. The optimal results were as follows: the sample concentration was 3.04 mg/mL, the ethanol concentration was 70.33 %, pH was 2.93, the elution velocity was 2.95 Bv/h, and the comprehensive score was 75.52 ± 0.53 % (n = 3). The purified flavonoids of CMK (PFCMK) were compared with a reference substance through ultra high-performance liquid chromatography and ultraviolet spectra. Results of composition analyses were as follows: (−)−gallocatechin, 5−Caffeoylquinic acid, (−)−naringin, myricetin, luteolin, and isorhamnetin. The IC₅₀ values of 2,2–dIphenyl–1-picrylhydrazyl (0.034 mg/mL), hydroxyl radical method (0.643 mg/mL), and reducing power (0.025 mg/mL) showed that PFCMK had strong antioxidant activity. The minimum inhibitory concentrations on Bacillus pumilus, Bacillus subtilis, and Escherichia coli were 1.75, 1.75, and 0.875 mg/mL, respectively. PFCMK can be prepared as an antioxidant and antimicrobial for natural pharmaceutical, cosmetic products, and other industrial products.

采用层次分析法（AHP）和响应面分析法（RSM）相结合，优化了Carex meyeriana Kunth（CMK）粗黄酮的纯化工艺。根据抗氧化剂和抗菌活性评估了类黄酮的生物活性。基于AHP的综合评分方法的加权系数包括回收率，纯度和脱色率。最佳结果如下：样品浓度为3.04 mg / mL，乙醇浓度为70.33％，pH为2.93，洗脱速度为2.95 Bv / h，综合评分为75.52±0.53％（n = 3）。通过超高效液相色谱和紫外光谱，将纯化的CMK黄酮（PFCMK）与参考物质进行比较。组成分析的结果如下：（-）-没食子儿茶素，5-咖啡酰奎宁酸，（-）-柚皮苷，杨梅素，木犀草素和异鼠李素。2,2–二苯基–1-甲基肼基（0.034 mg / mL），羟自由基法（0.643 mg / mL）和还原力（0.025 mg / mL）的IC ₅₀值表明，PFCMK具有很强的抗氧化活性。对短小芽孢杆菌，枯草芽孢杆菌和大肠杆菌的最小抑菌浓度分别为1.75、1.75和0.875 mg / mL。PFCMK可以用作天然药物，化妆品和其他工业产品的抗氧化剂和抗菌剂。