In this work, a novel dual-excitation and dual-emission fluorescent probe, CDs–COO–Fluorescein (CDs–COO–F), for the specific detection of hydrazine (N₂H₄) was designed and synthesized through esterification reaction of carbon dots (CDs) and fluorescein (F). The prepared CDs were characterized by TEM, XPS, XRD and FT-IR and the optical properties of CDs and CDs–COO–F were described by UV–vis and fluorescence spectra. On the other hand, the affecting factors including hydrazine (N₂H₄) concentration and interfering substances on the hydrazine (N₂H₄) detection were researched. The high sensitivity and high selectivity of CDs–COO–F in detection hydrazine (N₂H₄) could be confirmed. Two good linear responses of fluorescence emission intensities of CDs–COO–F at 462 nm and 515 nm as functions of hydrazine (N₂H₄) concentration were obtained, respectively. The limits of detection hydrazine (N₂H₄) were 0.504 μM and 0.269 μM under 370 nm and 480 nm excitation wavelength lights, respectively. The detecting mechanism of hydrazine (N₂H₄) by CDs–COO–F was deduced from the experimental results. Cell imaging and cytotoxicity experiments of CDs–COO–F were measured. The experiment results show that the fluorescent probe CDs–COO–F can be applied to detect hydrazine (N₂H₄) in aqueous solution and living cells.

在这项工作中，设计并通过碳的酯化反应合成了新颖的双激发双发射荧光探针CDs-COO-荧光素（CDs-COO-F），用于肼（N ₂ H ₄）的特异性检测。点（CD）和荧光素（F）。制备的CD通过TEM，XPS，XRD和FT-IR进行表征，并通过UV-vis和荧光光谱描述CD和CD-COO-F的光学性质。另一方面，研究了肼（N ₂ H ₄）浓度和干扰物质对肼（N ₂ H ₄）检测的影响因素。CD-COO-F在检测肼中的高灵敏度和高选择性（N ₂ H₄）可以确认。分别获得了在462 nm和515 nm处CDs–COO–F的荧光发射强度随肼（N ₂ H ₄）浓度变化的两个良好的线性响应。在370 nm和480 nm激发波长光下，检测肼（N ₂ H ₄）的极限分别为0.504μM和0.269μM。实验结果推导了CDs–COO–F对肼（N ₂ H ₄）的检测机理。测量了CDs–COO–F的细胞成像和细胞毒性实验。实验结果表明，荧光探针CDs–COO–F可用于检测肼（N ₂ H ₄）在水溶液和活细胞中。