In this study, we investigated rearing and breeding razor clam (Sinonovacula constricta) in inland alkaline brackish water. During 45 days of the experiment in candidate target inland area brackish water (TBW), survival, growth, enzyme activity and immune responses were analysed. The results showed that shell length and weight were significantly inhibited in the TBW group (p <0.001). Although the cumulative mortality was 81.479 ± 7.028 (p < 0.001) at 15 days, it increased slowly to 92.915 ± 2.271 (p < 0.001) at 45 days. The enzyme activities of Na⁺/K⁺-ATPase, aspartate aminotransferase and superoxide dismutase peaked at 15 days, and juvenile S. constricta (JSC) may engaged in partial anaerobic metabolism or damage to gill tissue, which may explain the high mortality rate at this time. Additionally, the phagocytic ability of haemocytes was inhibited (p < 0.001), but the metabolic activity was enhanced (p < 0.001). This implies that 15 days was the peak of TBW stress, and stress gradually decreased by days 30 and 45. TBW affected metabolism, osmotic regulation, and immune responses. There was an independent ionic interaction perhaps effect on JSC, primarily through Na⁺/K⁺ rate. Approximately 7 % of animals adapted successfully to TBW after 45 days. In summary, S. constricta has a great potential in further anti-TBW conditions selective breeding research.

在这项研究中，我们调查了内陆碱性微咸水中的剃刀蛤（Sinonovacula constricta）的饲养和繁殖。在45天的候选目标内陆微咸水（TBW）中，分析了其存活，生长，酶活性和免疫反应。结果表明，在TBW组中，壳长和重量受到了显着抑制（p <0.001）。尽管在15天时的累积死亡率为81.479±7.028（p <0.001），但在45天时却缓慢增加至92.915±2.271（p <0.001）。Na ⁺ / K ⁺ -ATPase，天冬氨酸转氨酶和超氧化物歧化酶的酶活性在15天达到峰值，而少年链球菌（JSC）可能参与部分厌氧代谢或对g组织的损害，这可能解释了此时的高死亡率。此外，血细胞的吞噬能力被抑制（p <0.001），但代谢活性增强（p <0.001）。这意味着15天是TBW应激的高峰，而应激在30天和45天时逐渐降低。TBW影响代谢，渗透调节和免疫反应。主要通过Na ⁺ / K ⁺速率，可能对JSC有独立的离子相互作用。45天后约有7％的动物成功适应了TBW。综上所述，缢有进一步的抗TBW条件选择育种研究的巨大潜力。