In this study, we investigated rearing and breeding razor clam () in inland alkaline brackish water. During 45 days of the experiment in candidate target inland area brackish water (TBW), survival, growth, enzyme activity and immune responses were analysed. The results showed that shell length and weight were significantly inhibited in the TBW group ( <0.001). Although the cumulative mortality was 81.479 ± 7.028 ( < 0.001) at 15 days, it increased slowly to 92.915 ± 2.271 ( < 0.001) at 45 days. The enzyme activities of Na/K-ATPase, aspartate aminotransferase and superoxide dismutase peaked at 15 days, and juvenile (JSC) may engaged in partial anaerobic metabolism or damage to gill tissue, which may explain the high mortality rate at this time. Additionally, the phagocytic ability of haemocytes was inhibited ( < 0.001), but the metabolic activity was enhanced ( < 0.001). This implies that 15 days was the peak of TBW stress, and stress gradually decreased by days 30 and 45. TBW affected metabolism, osmotic regulation, and immune responses. There was an independent ionic interaction perhaps effect on JSC, primarily through Na/K rate. Approximately 7 % of animals adapted successfully to TBW after 45 days. In summary, has a great potential in further anti-TBW conditions selective breeding research.

中文翻译：

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内陆碱性微咸水养殖蛏子幼苗：生存、生长、生理和免疫反应


在这项研究中，我们调查了内陆碱性微咸水中的蛏子（）的饲养和繁殖。在候选目标内陆地区苦咸水 (TBW) 中进行的 45 天实验中，对存活、生长、酶活性和免疫反应进行了分析。结果表明，TBW组的壳长和重量受到显着抑制（<0.001）。尽管15天时的累积死亡率为81.479±7.028（<0.001），但在45天时却缓慢增加至92.915±2.271（<0.001）。 Na/K-ATPase、天冬氨酸氨基转移酶和超氧化物歧化酶的酶活性在15天时达到峰值，幼鱼（JSC）可能正在进行部分无氧代谢或鳃组织受损，这可能解释了此时的高死亡率。此外，血细胞的吞噬能力受到抑制（<0.001），但代谢活性增强（<0.001）。这意味着15天是TBW应激的峰值，并且在第30天和45天应激逐渐下降。TBW影响新陈代谢、渗透调节和免疫反应。独立的离子相互作用可能对 JSC 产生影响，主要是通过 Na/K 比率。 45 天后，大约 7% 的动物成功适应 TBW。综上所述，进一步开展抗TBW条件选育研究具有巨大潜力。