Abstract

One of the promising directions in modern pharmacology is the creation of biologically active drugs based on substances of natural origin. Of particular interest in this regard are peptides of fish skin mucus. The aim of the work was to purify and isolate the protein fractions—thromboplastin (clotting factor III, tissue factor, TF) and prothromboplastin (clotting factor XI, F11) from fish skin mucus using affine and ion-exchange chromatography as well as mass spectrometry. The mucus of two fish species was used as the material for the study: the African clarias catfish (Clarias gariepinus Burchell, 1822) and the carp (Cyprinus carpio carpio Linnaeus, 1758). Among the identified proteins, it was not possible to find proteins that have similar or highly homologous amino acid sequences with human thromboplastin and prothromboplastin. However, given the ability of fish skin mucus to stimulate coagulation, as well as the ability of individual fractions to interact with antibodies to thromboplastin and prothromboplastin, it can be argued that the identified proteins have epitopes that are homologous in function to thromboplastin or prothromboplastin, thanks to which components of fish skin mucus can reduce the time of coagulation.

中文翻译：

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鱼皮粘液具有血栓形成活性的蛋白质成分研究

摘要

现代药理学中有希望的方向之一是基于天然物质的生物活性药物的开发。在这方面特别感兴趣的是鱼皮粘液的肽。这项工作的目的是使用仿射和离子交换色谱以及质谱技术从鱼皮粘液中纯化和分离蛋白质组分-凝血活酶（凝血因子III，组织因子，TF）和凝血活酶（凝血因子XI，F11）。 。两种鱼类的粘液用作研究的材料：非洲the鱼（Clarias gariepinus Burchell，1822年）和鲤鱼（Cyprinus carpio carpio林奈（1758）。在已鉴定的蛋白质中，不可能找到与人凝血活酶和凝血活酶原具有相似或高度同源的氨基酸序列的蛋白质。但是，鉴于鱼皮粘液具有刺激凝血的能力，以及各个组分与抗凝血酶原和促凝血酶原原蛋白的抗体相互作用的能力，可以说，鉴定出的蛋白质具有与凝血酶原或促凝血酶原原蛋白功能相同的表位，由于鱼皮粘液的哪些成分可以减少凝血时间。