Abstract

Comparative evaluation of three different methods for assessing stallion sperm-membrane integrity, including the eosin staining technique, the fluorochrome staining (SYBR-14 + PI) method, and the hypo-osmotic test, has been made in order to reveal the most efficient method of measurement for this item. The stallion sperm showed high coefficients of correlation between sperm motility and intact membrane proportion with all the methods of measurement. The best coincidence result was attained with the eosin staining technique, assessed by a correlation coefficient of 0.91. This technique has the advantages of simplicity, accessibility, and efficiency. Accurate assessment requires performing a method of subtracting a number of unstained damaged sperm cells, which are nonfunctional, from a total number of unstained spermatozoa. The proportion of motile sperm does not coincide with the proportion of sperm intact membranes during the stallion-semen storage process according to all the methods of measurement. A significant sperm count remains with intact cell membranes at the loss of total motility at the end of a storage process. This indicates that the sperm cells become immotile because of layout of the energetic material in flagellar mitochondria, which is not associated with membrane integrity. Therefore, it is recommended to assess the stallion sperm membranes with the use of fresh or thawed sperm. It is suggested to use assessment of membrane integrity in clarifying the sperm motility, particularly, in especially doubtful cases.

中文翻译：

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种马精子膜完整性评估方法的比较评价

摘要

为了揭示最有效的方法，已对三种不同的评估种马精子细胞膜完整性的方法进行了比较评估，包括曙红染色技术，荧光染色（SYBR-14 + PI）方法和低渗渗透测试。此项目的测量值。使用所有测量方法，种马精子在精子活力和完整膜比例之间都显示出很高的相关系数。曙红染色技术获得了最佳的重合结果，相关系数为0.91。该技术具有简单，可访问和高效的优点。准确的评估需要执行一种从未染色的精子总数中减去许多未染色的受损精子细胞的方法。根据所有测量方法，在种马精液存储过程中，能动精子的比例与完整子精子的比例不一致。在储存过程结束时，完整的细胞膜仍保留着大量的精子，而这些细胞的完整运动力丧失了。这表明，由于鞭毛线粒体中高能物质的排列，精子细胞变得不活动，这与膜的完整性无关。因此，建议使用新鲜或解冻的精子评估种马精子膜。建议使用膜完整性评估来澄清精子运动，特别是在特别可疑的情况下。