Introduction

Rice leaves and stems, which can be used as rice straw for livestock feed, accumulate soluble oxalate. The oxalate content often reaches 5% of the dry weight leaves. Excess uptake of oxalate-rich plants causes mineral deficiencies in vertebrates, so it is important to reduce the oxalate content in rice leaves to produce high-quality rice straw. However, the mechanism of oxalate accumulation in rice has remained unknown.

Objectives

To understand metabolic networks relating oxalate accumulation in rice.

Methods

In this study, we performed metabolome analysis of rice M₂ population generated by ion-beam irradiation using CE-MS.

Results

The result showed wide variation of oxalate contents in M₂ plants compared with those of control plants. Multivariate analyses of metabolome dataset revealed that oxalate accumulation was strongly related with anionic compounds such as 2OG and succinate. For low-oxalate plants, four patterns of metabolic alterations affected oxalate contents in the M₂ leaves were observed. In M₃ plants, we found putative low-oxalate line obtained from low-oxalate M₂ mutant.

Conclusions

These findings would lead to produce the low-oxalate rice and to understand the oxalate synthesis in plants.These findings would lead to produce the low-oxalate rice and to understand the oxalate synthesis in plants.

中文翻译：

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水稻叶片的代谢组分析，以从离子束突变种群获得低草酸盐菌株。

介绍

可用作农作物饲料的稻草的稻叶和茎，会积累可溶性草酸盐。草酸含量通常达到干重叶的5％。富含草酸盐的植物摄入过多会导致脊椎动物缺乏矿物质，因此，重要的是减少稻米叶片中的草酸盐含量，以生产高质量的稻草。然而，草酸在水稻中积累的机理仍然未知。

目标

了解与稻米中草酸盐积累有关的代谢网络。

方法

在这项研究中，我们使用CE-MS对离子束辐照产生的水稻M ₂种群进行了代谢组学分析。

结果

结果显示与对照植物相比，M ₂植物中草酸盐含量的变化很大。代谢组数据集的多变量分析表明，草酸盐的积累与阴离子化合物（如2OG和琥珀酸酯）密切相关。对于低草酸盐植物，观察到四种代谢变化模式影响了M ₂叶片中的草酸盐含量。在M ₃植物中，我们发现了从低草酸盐M ₂突变体获得的推定的低草酸盐品系。

结论

这些发现将导致产生低草酸盐的水稻并了解植物中的草酸盐合成。这些发现将导致产生低草酸盐的水稻并了解植物中的草酸盐合成。