当前位置: X-MOL 学术J. Nanopart. Res. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Cytotoxicity of pristine and functionalized tungsten disulfide particles in the urinary system
Journal of Nanoparticle Research ( IF 2.1 ) Pub Date : 2020-09-07 , DOI: 10.1007/s11051-020-05007-1
Lorena Garcia-Hevia , Irene Roehrer , Tommaso Mazzocchi , Arianna Menciassi , Leonardo Ricotti

Tungsten disulfide (WS2) particles have excellent lubrication properties and low surface energy. These features are exploited for numerous applications in the biomedical field, from the development of low-friction orthodontic wires to urinary encrustation-resisting coatings. Nevertheless, the cytotoxicity of spherical WS2 particles still remains unclear. We report the biocompatibility of pristine WS2 particles in human urinary bladder cells after the exposure to a wide range of concentrations (from 0 to 100 μg/mL). Based on the cell viability assessments using trypan blue exclusion assay and the PrestoBlue Cell viability reagent after 24, 48, and 72 h exposure, it can be concluded that these particle aggregates, with a diameter of ~ 500 nm, do not show any evidence of alterations or toxicity in the cells, even with high concentrations there exist a 87% of cell viability. Furthermore, due to the predisposition to agglomerate of this material and the possible use as a coating in urinary devices, we functionalized these particles with carboxylic groups. Aggregates with a diameter of ~ 370 nm were obtained. Zeta potential measurements highlighted that carboxylation correctly occurred, showing a switch from ~ + 4 for pristine particles to ~ − 35 for COOH-functionalized ones. Cytotoxic studies with these functionalized WS2 particles also showed good biocompatibility on human urinary bladder cells, with an 86% of viability after incubation with 100 μg/mL of particles over 72 h. These results highlight a good potential of WS2 particles for a future safe application as components of implantable medical devices.



中文翻译:

原始和功能化二硫化钨颗粒在泌尿系统中的细胞毒性

二硫化钨(WS 2)颗粒具有出色的润滑性能和低表面能。从开发低摩擦正畸丝到抗尿结石涂层,这些功能已在生物医学领域得到了广泛应用。尽管如此,球形WS 2颗粒的细胞毒性仍然不清楚。我们报道了原始WS 2的生物相容性暴露于多种浓度(0至100μg/ mL)的人膀胱细胞中的颗粒。根据使用锥虫蓝排除试验和PrestoBlue Cell活力试剂在暴露24、48和72 h后的细胞活力评估,可以得出结论,这些直径约500 nm的颗粒聚集体没有任何证据表明即使在高浓度的情况下,细胞中的改变或毒性也会存在87%的细胞活力。此外,由于这种材料易于团聚,并有可能用作泌尿设备的涂层,因此我们用羧基官能化了这些颗粒。获得了直径约为370 nm的聚集体。Zeta电势测量结果表明羧基正确地发生了,显示原始颗粒的〜+ 4变为COOH-官能化颗粒的〜-35。这些功能化WS的细胞毒性研究2个颗粒在人的膀胱细胞上也表现出良好的生物相容性,与100μg/ mL颗粒孵育72小时后的活力为86%。这些结果凸显了WS 2颗粒作为植入式医疗设备组件的未来安全应用的巨大潜力。

更新日期:2020-09-08
down
wechat
bug