Abstract

Long noncoding RNAs (lncRNAs) are promising biomarkers and potential targets for liver cancer therapy. Stable hepatocyte lines are used in vitro to investigate functions of lncRNAs which amount in cell fluctuates during carcinogenesis. For the first time we compared gene expression of known lncRNAs in human conditional normal liver cells HepaRG and cancer cell lines Huh7 and HepG2. We showed that relative amounts of these lncRNAs in HepaRG are close to analogous variables measured for liver samples from healthy donors. Obtained data demonstrate exclusive peculiarities of HepaRG and confirm its reasonable application as a model of normal human hepatocytes for studying functions of lncRNAs.

中文翻译：

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人肝细胞细胞系和肝脏中长非编码RNA表达的比较分析。

摘要

长的非编码RNA（lncRNA）是有前途的生物标志物和肝癌治疗的潜在目标。在体外使用稳定的肝细胞系来研究lncRNA的功能，该功能在癌变过程中会发生细胞波动。我们首次比较了人类条件正常肝细胞HepaRG和癌细胞系Huh7和HepG2中已知lncRNA的基因表达。我们表明，在HepaRG中这些lncRNA的相对量接近于健康供体肝脏样品的类似变量。获得的数据证明了HepaRG的独特特性，并证实了其作为正常人肝细胞模型研究lncRNA功能的合理应用。