Abstract

The possibility to visualize small bacterial RNAs inside macrophages infected with Mycobacterium tuberculosis was demonstrated for the first time. A macrophage cell line was infected with the M. tuberculosis strain expressing small noncoding mycobacterial RNA MTS1338 fused with an RNA aptamer, which could bind a fluorophore and trigger its fluorescence. As a result, treatment of the infected macrophages with the DFHBI-1T fluorophore allowed fluorescence-based detection of the aptamer-labeled MTS1338 both in mycobacteria and in the host cell cytoplasm. This system can significantly aid in revealing the role of small M. tuberculosis RNAs in the pathogenesis of tuberculosis through identification of their secretion routes and eukaryotic targets and elucidation of the associated molecular pathways.

中文翻译：

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基因编码的荧光适体在感染的巨噬细胞中结核分枝杆菌小RNA MTS1338可视化中的应用。

摘要

首次展示了可视化结核分枝杆菌感染的巨噬细胞内小细菌RNA的可能性。巨噬细胞细胞系感染了结核分枝杆菌菌株，该菌株表达与RNA适体融合的小的非编码分枝杆菌小RNA MTS1338，它可以结合荧光团并触发其荧光。结果，用DFHBI-1T荧光团处理感染的巨噬细胞可以在分枝杆菌和宿主细胞质中进行基于荧光的适体标记的MTS1338检测。该系统可以显着帮助揭示小结核分枝杆菌的作用 通过鉴定结核菌的分泌途径和真核靶标以及阐明相关的分子途径，来研究结核病的发病机理中的RNA。