The medicinal mushroom Ophiocordyceps sobolifera has been used in traditional Chinese medicine; however, the proven medical benefits of the crude protein are limited. Therefore, in this study, forty-two isolates of O. sobolifera were identified using their internal transcribed spacer (ITS) sequences. Then, the antibacterial activities of the crude proteins were evaluated against strains of methicillin-resistant and -susceptible Staphylococcus aureus and Bacillus cereus using agar well diffusion technique. The results revealed that the crude protein had a good inhibitory effect against S. aureus and B. cereus. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values of the crude proteins ranged from 0.156–10.00 mg/mL and 0.156–10.00 mg/mL, respectively. Among them, the Cod-NB1302 strain had the broadest inhibitory activity against the tested bacterial pathogens with MIC values of 0.625–1.25 mg/mL and MBC values of 0.625–1.25 mg/mL. Then, the species-specific molecular marker derived from random amplified polymorphic DNA (RAPD) was developed for the identification of O. sobolifera. The results indicated that the OPT13 arbitrary primer was able to generate two species-specific DNA fragments from O. sobolifera. Specific fragments were then cloned, sequenced, and used to design the species-specific primers. Only one species-specific primer set, the SpsoL_f01/SpsoL_r01 primer pair, produced a prominent amplicon of 118 bps in O. sobolifera under stringent conditions without non-specific detection after being applied to other species, with the exception of O. longissima. Moreover, the detection limit of this primer was 5.84 ng of genomic DNA, and this primer set could be applied in all strains of O. sobolifera.

Sobolifera药用蘑菇Ophiocordyceps已在中药中使用。但是，粗蛋白的证明的医学益处是有限的。因此，在这项研究中，使用其内部转录间隔区（ITS）序列鉴定了42个Sobolifera分离株。然后，使用琼脂孔扩散技术评估粗蛋白对耐甲氧西林和易感金黄色葡萄球菌和蜡状芽孢杆菌的菌株的抗菌活性。结果表明，该粗蛋白对金黄色葡萄球菌和蜡状芽孢杆菌具有良好的抑制作用。。粗蛋白的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）值分别为0.156-10.00 mg / mL和0.156-10.00 mg / mL。其中，Cod-NB1302菌株对被测细菌病原体的抑制作用最广，MIC值为0.625–1.25 mg / mL，MBC值为0.625–1.25 mg / mL。然后，开发了一种来自随机扩增多态性DNA（RAPD）的物种特异性分子标记物，用于鉴定so。sobolifera。结果表明，OPT13任意引物能够从sobolifera产生两个物种特异性的DNA片段。然后将特定片段克隆，测序并用于设计物种特异性引物。只有一个物种特异性引物组，所述SpsoL_f01 / SpsoL_r01引物对，制备在118个bps的突出的扩增子O. sobolifera被应用到其它物种，具有的异常后在严格条件下不非特异性检测O.椰心叶甲。此外，该引物的检测极限是5.84ng的基因组DNA，并且该引物组可以应用于所有的O. sobolifera菌株。