Foot-and-mouth disease (FMD) is a highly contagious disease that affects cattle, sheep, goats, pigs, and over 70 species of wildlife. FMD continues to be a major economic concern for livestock productivity in many countries. FMDV has seven serotypes O, A, Asia 1, C, and Southern Africa Territories (SAT) 1, 2, and 3. Although SAT 1, and SAT 3 outbreaks are not as common as serotypes O, A, Asia 1, and SAT 2, outbreaks have also been reported. The recent outbreaks of SAT 1 occurred in Cameroon, Zimbabwe, South Africa, and Uganda, while most recent SAT 3 occurred in Namibia in 2019. The development of rapid and easy-to-perform FMDV detection tests is critical to control the outbreak and spread of FMD.

The current project has produced monoclonal antibodies (mAb) against FMDV serotypes SAT 1, and SAT 3. Using these mAbs, two lateral flow immunochromatographic (LFI) strip tests for the detection of FMDV SAT 1, and SAT 3 have been developed. SAT 1 strip test detected 14 out of 15 SAT 1 field isolates. The SAT 3 strip test detected all four SAT 3 isolates tested, but the signal is weak for UGA 10/97 and showed no cross-reactivity with other FMDV serotypes. The diagnostic specificities of the SAT 1 and the SAT 3 tests are 100%, which are higher than double antibody sandwich (DAS) ELISA. The diagnostic sensitivity of the SAT 1 test strip is lower than that of DAS ELISA, while the diagnostic sensitivity of the SAT 3 test strip is similar to that of DAS ELISA. The first reported SAT 1 and SAT 3 strip test combined with the previously developed SAT 2 strip test can be used for quick diagnosis in endemic countries in Africa. Rapid identification of FMDV serotypes is critical for disease control and vaccine selection. Also, these strip tests can be used in the laboratory to quickly screen samples from the field.

口蹄疫 (FMD) 是一种高度传染性疾病，会影响牛、绵羊、山羊、猪和 70 多种野生动物。在许多国家，口蹄疫仍然是影响牲畜生产力的主要经济问题。FMDV 有七种血清型 O、A、亚洲 1、C 和南部非洲领土 (SAT) 1、2 和 3。虽然 SAT 1 和 SAT 3 的爆发不如血清型 O、A、亚洲 1 和 SAT 2、也有暴发的报道。最近的 SAT 1 爆发发生在喀麦隆、津巴布韦、南非和乌干达，而最近的 SAT 3 爆发发生在 2019 年的纳米比亚。 开发快速且易于执行的口蹄疫病毒检测测试对于控制疫情和传播至关重要口蹄疫。

目前的项目已经生产了针对 FMDV 血清型 SAT 1 和 SAT 3 的单克隆抗体 (mAb)。使用这些 mAb，开发了两种用于检测 FMDV SAT 1 和 SAT 3 的侧流免疫色谱 (LFI) 条带测试。SAT 1 条带测试检测到 15 个 SAT 1 现场分离物中的 14 个。SAT 3 试纸检测检测到所有四种 SAT 3 分离株，但 UGA 10/97 的信号较弱，并且与其他 FMDV 血清型没有交叉反应。SAT 1 和 SAT 3 测试的诊断特异性为 100%，高于双抗体夹心 (DAS) ELISA。SAT 1试纸的诊断灵敏度低于DAS ELISA，而SAT 3试纸的诊断灵敏度与DAS ELISA相似。首次报道的 SAT 1 和 SAT 3 试纸结合先前开发的 SAT 2 试纸可用于非洲流行国家的快速诊断。快速鉴定口蹄疫病毒血清型对于疾病控制和疫苗选择至关重要。此外，这些条带测试可用于实验室快速筛选现场样品。