Reversible glycosylation polypeptide (RGP) is a type of plant-specific protein, primarily involved in the biosynthesis of cell wall polysaccharides, which in turn changes the shape of the cell walls and affects the wood properties of plants. Poplar is a major industrial timber species, and the RGP gene has not been studied. This study uses bioinformatics methods to predict physical and chemical characters such as molecular weight, isoelectric point, and hydrophilicity; and fluorescent quantitative method to determine the effect of different forms of nitrogen on the transcription level of the gene family. The results showed that there are six RGP homologous genes in the Populus trichocarpa genome, which were distributed on the six chromosomes of P. trichocarpa. The family members have a simple gene structure and contain four exons and introns. Phylogenetic tree analysis showed that RGP genes all belong to Class I in P. trichocarpa. Tissue-specific expression analysis showed that PtRGP1 and PtRGP2 were highly expressed in the stems, PtRGP4 and PtRGP5 were highly expressed in the upper leaves, PtRGR3 and PtRGR6 were expressed in stems and internodes, but the relative expression is not high. Quantitative real-time RT-PCR (qRT-PCR) analyses revealed that PtRGP3 and 6 were up-regulated in the upper stem in response to the low ammonium and high nitrate treatments. The influence of nitrogen on the expression of PtRGP3 and 6 genes may affect the formation of the plant secondary cell wall. This study lays a foundation for further study on the function of RGP genes in P. trichocarpa.

可逆糖基化多肽（RGP）是一种植物特异性蛋白，主要参与细胞壁多糖的生物合成，进而改变细胞壁的形状，影响植物的木材特性。杨树是主要的工业用材树种，RGP基因尚未研究。本研究使用生物信息学方法预测分子量、等电点和亲水性等物理和化学特性；和荧光定量方法确定不同形式的氮对基因家族转录水平的影响。结果表明，毛果杨基因组中有6个RGP同源基因，分布在6条染色体上。P. trichocarpa。该家族成员基因结构简单，包含四个外显子和内含子。系统进化树分析表明，RGP基因在毛果松中均属于I类。组织特异性表达分析表明，PtRGP1和PtRGP2在茎中高表达，PtRGP4和PtRGP5在上部叶片中高表达，Pt R GR3和Pt R GR6在茎和节间表达，但相对表达量不高。定量实时 RT-PCR (qRT-PCR) 分析显示PtRGP3和6响应于低铵和高硝酸盐处理，在上部茎中上调。氮对PtRGP3和6基因表达的影响可能影响植物次生细胞壁的形成。本研究为进一步研究赤果松RGP基因的功能奠定了基础。