Abstract

Polyurethanes are widely used in interventional devices due to the excellent physicochemical property. However, non-specific adhesion and severe inflammatory response of ordinary polyurethanes may lead to severe complications of intravenous devices. Herein, a novel phospholipid-based polycarbonate urethanes (PCUs) were developed via two-step solution polymerization by direct synthesis based on functional raw materials. Furthermore, PCUs were coated on biomedical metal sheets to construct biomimetic anti-fouling surface. The results of stress–strain curves exhibited excellent tensile properties of PCUs films. Differential scanning calorimetry results indicated that the microphase separation of such PCUs polymers could be well regulated by adjusting the formulation of chain extender, leading to different biological response. In vitro blood compatibility tests including bovine serum albumin adsorption, fibrinogen adsorption and denaturation, platelet adhesion and whole-blood experiment showed superior performance in inhibition non-specific adhesion of PCUs samples. Endothelial cells and smooth muscle cells culture tests further revealed a good anti-cell adhesion ability. Finally, animal experiments including ex vivo blood circulation and subcutaneous inflammation animal experiments indicated a strong ability in anti-thrombosis and histocompatibility. These results high light the strong anti-adhesion property of phospholipid-based PCUs films, which may be applied to the blood-contacting implants such as intravenous catheter or antithrombotic surface in the future.

中文翻译：

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磷脂基聚碳酸酯氨基甲酸酯的制备，可用于血液接触型植入物的潜在应用

摘要

聚氨酯由于其出色的理化特性而被广泛用于介入设备。但是，普通聚氨酯的非特异性粘附和严重的炎症反应可能导致静脉内器械严重并发症。在这里，一种新型的磷脂基聚碳酸酯氨基甲酸酯（PCU）是通过基于功能原料的直接合成两步溶液聚合。此外，将PCU涂覆在生物医学金属板上以构建仿生防污表面。应力-应变曲线的结果显示了PCU薄膜的优异拉伸性能。差示扫描量热法的结果表明，通过调节扩链剂的配方可以很好地调节此类PCUs聚合物的微相分离，从而导致不同的生物学反应。体外血液相容性测试（包括牛血清白蛋白吸附，纤维蛋白原吸附和变性，血小板粘附和全血实验）显示出优异的抑制PCU样品非特异性粘附的性能。内皮细胞和平滑肌细胞的培养试验进一步揭示了良好的抗细胞粘附能力。最后，包括离体血液循环和皮下炎症在内的动物实验表明，其抗血栓形成和组织相容性的能力很强。这些结果突出显示了基于磷脂的PCU薄膜的强抗粘附性能，该薄膜将来可能会应用于与血液接触的植入物，例如静脉内导管或抗血栓表面。