Rupture of Abdominal aortic aneurysm (AAA) is among the 15 leading causes of death after age 65. Using high frequency ultrasound, we showed that photobiomodulation (PBM) prevents formation and progression of AAA in the angiotensin‐II (Ang‐II)‐infused, apolipoprotein‐e‐deficient mouse model. In the current study we report that while challenge of porcine aortic Smooth Muscle Cells (SMCs) with Ang‐II (1 μM) resulted in a marked decay in mitochondrial membrane potential (MitMP) vs non‐challenged cells, treatment with PBM (continuous diode laser, 780 nm, 6.7 mW/cm², 5 minutes, 2 J/cm²) or pre‐incubation with estrogen (50 nM, 1 hour) significantly attenuated this deterioration in MitMP. We also report that PBM and estrogen markedly affected porcine aortic SMC contraction and modified mitochondrial dispersion reflecting important influence on SMC function. These studies provide strong evidence of the important underlying role of mitochondria in the preventive effect of PBM on formation and progression of AAA and its reduced incidence and delayed onset in women.

中文翻译：

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光生物调节和雌激素稳定血管紧张素II攻击猪主动脉平滑肌细胞中的线粒体膜电位。

腹主动脉瘤破裂（AAA）是65岁后死亡的15个主要死因之一。使用高频超声，我们证明了光生物调节（PBM）阻止了注入血管紧张素II（Ang-II）的AAA的形成和进展。 ，载脂蛋白不足的小鼠模型。在当前的研究中，我们报道了用Ang-II（1μM）攻击猪主动脉平滑肌细胞（SMC）会导致线粒体膜电位（MitMP）相对于未挑战的细胞显着下降，而PBM（连续二极管）处理激光，780nm时，6.7毫瓦/厘米²，5分钟，2 J /厘米²）或与雌激素预孵育（50 nM，1小时）可以显着减轻MitMP的这种恶化。我们还报告说，PBM和雌激素显着影响猪主动脉SMC收缩和修饰的线粒体分散，反映了对SMC功能的重要影响。这些研究提供了线粒体在PBM预防AAA形成和发展以及降低女性发病率和延缓发病中的重要潜在作用的有力证据。