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Submerged fermentation of Ginkgo biloba seeds powder using Eurotium cristatum for the development of ginkgo seeds fermented products
Journal of the Science of Food and Agriculture ( IF 3.3 ) Pub Date : 2020-09-06 , DOI: 10.1002/jsfa.10792
Minmin Zou 1, 2 , Wen Zhang 1, 2 , Qihui Dong 1, 2 , Chao Tang 1 , Fuliang Cao 2 , Erzheng Su 1, 2, 3
Affiliation  

BACKGROUND Ginkgo biloba seeds are well known for the significant curative effects on relieving cough and asthma. However, the development of ginkgo seeds products still falls behind at present, resulting in a great waste of ginkgo seeds resource. In this work, submerged fermentation of ginkgo seeds powder using Eurotium Cristatum was studied to investigate its feasibility as a new processing method. RESULTS To promote the growth of E. cristatum, the optimum fermentation medium was 80.0 g L-1 ginkgo seeds powder with addition of 5.0 g L-1 CaCl2 , 4.0 g L-1 MgSO4 , 1.25 g L-1 ZnSO4 and 0.65 g L-1 FeSO4 . The optimum fermentation conditions were pH 5.8 ± 0.1, inoculum size 5.1 × 106 CFU mL-1 , liquid medium volume 100 mL in 250-mL Erlenmeyer flask and fermentation 4 days. Through fermentation, the production of lovastatin in fermentation broth could reach up to 32.97 ± 0.17 μg mL-1 and the total antioxidant capacity was improved by more than two times. In addition, 40.15% of the ginkgotoxin in ginkgo seeds powder was degraded while the entire degradation of ginkgolic acids was obtained. Moreover, fermented ginkgo seeds powder suspension presented pleasant fragrances, and the activities of amylase and protease were enhanced to 11.30 ± 0.10 U mL-1 and 23.01 ± 0.20 U mL-1 , respectively. CONCLUSIONS Submerged fermentation using E. cristatum could significantly enhance the functional value and safety of ginkgo seeds powder, and had great potential to become a novel processing method for the development of ginkgo seeds fermented products. This article is protected by copyright. All rights reserved.

中文翻译:

银杏种子粉深层发酵利用冠麴菌开发银杏种子发酵产品

背景技术银杏种子以其显着的止咳平喘疗效而著称。然而,目前银杏种子产品的开发仍然滞后,造成了银杏种子资源的极大浪费。在这项工作中,研究了使用 Eurtium Cristatum 对银杏种子粉进行深层发酵,以研究其作为一种新加工方法的可行性。结果 为促进冠脉生长,最佳发酵培养基为80.0 g L-1银杏籽粉,添加5.0 g L-1 CaCl2、4.0 g L-1 MgSO4、1.25 g L-1 ZnSO4和0.65 g L -1 FeSO4 。最佳发酵条件为pH 5.8±0.1,接种量5.1×106 CFU mL-1,250mL锥形瓶中液体培养基体积100mL,发酵4天。通过发酵,发酵液中洛伐他汀的产量可达32.97±0.17 μg mL-1,总抗氧化能力提高2倍以上。此外,银杏籽粉中40.15%的银杏毒素被降解,同时获得了银杏酸的全部降解。此外,发酵的银杏种子粉末悬浮液呈现出令人愉悦的香味,淀粉酶和蛋白酶的活性分别提高到 11.30 ± 0.10 U mL-1 和 23.01 ± 0.20 U mL-1 。结论 冠花深层发酵可显着提高银杏籽粉的功能价值和安全性,具有成为开发银杏籽发酵产品的新型加工方法的潜力。本文受版权保护。版权所有。17 μg mL-1,总抗氧化能力提高两倍以上。此外,银杏籽粉中40.15%的银杏毒素被降解,同时获得了银杏酸的全部降解。此外,发酵的银杏种子粉末悬浮液呈现出令人愉悦的香味,淀粉酶和蛋白酶的活性分别提高到 11.30 ± 0.10 U mL-1 和 23.01 ± 0.20 U mL-1 。结论 冠花深层发酵可显着提高银杏籽粉的功能价值和安全性,具有成为开发银杏籽发酵产品的新型加工方法的潜力。本文受版权保护。版权所有。17 μg mL-1,总抗氧化能力提高两倍以上。此外,银杏籽粉中40.15%的银杏毒素被降解,同时获得了银杏酸的全部降解。此外,发酵的银杏种子粉末悬浮液呈现出令人愉悦的香味,淀粉酶和蛋白酶的活性分别提高到 11.30 ± 0.10 U mL-1 和 23.01 ± 0.20 U mL-1 。结论 冠花深层发酵可显着提高银杏籽粉的功能价值和安全性,具有成为开发银杏籽发酵产品的新型加工方法的潜力。本文受版权保护。版权所有。银杏籽粉中15%的银杏毒素被降解,而银杏酸完全降解。此外,发酵的银杏种子粉末悬浮液呈现出令人愉悦的香味,淀粉酶和蛋白酶的活性分别提高到 11.30 ± 0.10 U mL-1 和 23.01 ± 0.20 U mL-1 。结论 冠花深层发酵可显着提高银杏籽粉的功能价值和安全性,具有成为开发银杏籽发酵产品的新型加工方法的潜力。本文受版权保护。版权所有。银杏籽粉中15%的银杏毒素被降解,而银杏酸完全降解。此外,发酵的银杏种子粉末悬浮液呈现出令人愉悦的香味,淀粉酶和蛋白酶的活性分别提高到 11.30 ± 0.10 U mL-1 和 23.01 ± 0.20 U mL-1 。结论 冠脉深层发酵可显着提高银杏籽粉的功能价值和安全性,具有成为开发银杏籽发酵产品的新型加工方法的潜力。本文受版权保护。版权所有。分别为 30 ± 0.10 U mL-1 和 23.01 ± 0.20 U mL-1。结论 冠花深层发酵可显着提高银杏籽粉的功能价值和安全性,具有成为开发银杏籽发酵产品的新型加工方法的潜力。本文受版权保护。版权所有。分别为 30 ± 0.10 U mL-1 和 23.01 ± 0.20 U mL-1。结论 冠花深层发酵可显着提高银杏籽粉的功能价值和安全性,具有成为开发银杏籽发酵产品的新型加工方法的潜力。本文受版权保护。版权所有。
更新日期:2020-09-06
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