Determination of patulin in apple juice by amine‐functionalized solid‐phase extraction coupled with isotope dilution liquid chromatography tandem mass spectrometry,Journal of the Science of Food and Agriculture

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Determination of patulin in apple juice by amine‐functionalized solid‐phase extraction coupled with isotope dilution liquid chromatography tandem mass spectrometry
Journal of the Science of Food and Agriculture ( IF 4.1 ) Pub Date : 2020-09-17 , DOI: 10.1002/jsfa.10790
Xianjiang Li ₁ , Wen Ma ₂ , Qinghe Zhang ₁ , Hongmei Li ₁ , Huwei Liu ₃

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BACKGROUND Patulin is a mycotoxin that mainly contaminates apple juice which is a typical high sugar matrix. Many different adsorbents have been utilized to develop sample pretreatment method to minimize the matrix interferences from apple juice. RESULTS A simple and reliable extraction method for the determination of patulin in apple juice has been developed. The sample preparation involves a simple one-step cleanup procedure using amine-functionalized polymeric solid-phase extraction cartridges (Retain AX) that effectively removes interferences and facilitates the quantification. Isotope dilution liquid chromatography tandem mass spectrometry (ID-LC-MS/MS) was employed to minimize matrix effect and ensure reliable results. The limit of detection was as low as 0.2 μg/kg, which was satisfactory with current European USA and Chinese legislation. Matrix-matched linearity (r2 =0.9997) was established in the range of 1-400 μg/kg. Recovery was performed in samples spiked at three levels, and results were between 97.2% and 100.2%. CONCLUSION The results indicated that, with one-step cleanup, the matrix effect was negligible and sensitivity was satisfactory. Our work provided a simple and reliable method for patulin detection in apple juice and is also very promising in routine quality control. This article is protected by copyright. All rights reserved.

更新日期：2020-09-17

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