Mangiferin is a naturally occurring polyphenol, widely distributed in Thymeraceae families, and presents pharmacological activity, including anti‐cancer activities in many human cancer cell lines. Mangiferin has also been reported to affect immune responses; however, no available information concerning the effects of mangiferin on immune reactions in leukemia mice in vivo. In the present study, we investigated the effects of mangiferin on leukemia WEHI‐3 cell generated leukemia BLAB/c mice. Overall, the experiments were divided into two parts, one part was immune responses experiment and the other was the survival rate experiment. The immune responses and survival rate study, 40 mice for each part, were randomly separated into five groups (N = 8): Group I was normal animals and groups II‐V WEHI‐3 cell generated leukemia mice. Group II mice were fed normal diet as a positive control; group III, IV, and V mice received mangiferin at 40, 80, and 120 mg/kg, respectively, by intraperitoneal injection every 2 days for 20 days. Leukocytes cell population, macrophage phagocytosis, and NK cell activities were analyzed by flow cytometry. Isolated splenocytes stimulated with lipopolysaccharide (LPS) and concanavalin A (Con A) were used to determine the proliferation of B and T cells, respectively, and subsequently were analyzed by flow cytometry. Results indicated that mangiferin significantly increased body weight, decreased the liver and spleen weights of leukemia mice. Mangiferin also increased CD3 T‐cell and CD19 B cell population but decreased Mac‐3 macrophage and CD11b monocyte. Furthermore, mangiferin decreased phagocytosis of macrophages from PBMC and peritoneal cavity at 40, 80, and 120 mg/kg treatment. However, it also increased NK cell activity at 40 and 120 mg/kg treatment. There were no effects on T and B cell proliferation at three examined doses. In survival rate studies, mangiferin significantly elevated survival rate at 40 and 120 mg/kg treatment of leukemia mice in vivo.

中文翻译：

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芒果苷诱导免疫反应并评估 WEHI ‐3 细胞体内产生的小鼠白血病的存活率

芒果苷是一种天然存在的多酚，广泛分布于百里香科，具有药理活性，包括在许多人类癌细胞系中具有抗癌活性。据报道，芒果苷也会影响免疫反应。然而，尚无关于芒果苷对白血病小鼠体内免疫反应影响的信息。在本研究中，我们研究了芒果苷对白血病 WEHI-3 细胞产生的白血病 BLAB/c 小鼠的影响。总体来说，实验分为两部分，一部分是免疫反应实验，另一部分是存活率实验。免疫反应和存活率研究，每部分40只小鼠，随机分为五组（N = 8）：I组为正常动物，II-V组为WEHI-3细胞产生的白血病小鼠。II组小鼠正常饮食作为阳性对照；III、IV和V组小鼠分别接受芒果苷40、80和120 mg/kg，每2天腹腔注射一次，持续20天。通过流式细胞仪分析白细胞细胞群、巨噬细胞吞噬作用和 NK 细胞活性。用脂多糖 (LPS) 和刀豆球蛋白 A (Con A) 刺激的分离脾细胞分别用于测定 B 细胞和 T 细胞的增殖，随后通过流式细胞术进行分析。结果表明，芒果苷显着增加了白血病小鼠的体重，降低了肝脏和脾脏的重量。芒果苷还增加了 CD3 T 细胞和 CD19 B 细胞数量，但减少了 Mac-3 巨噬细胞和 CD11b 单核细胞。此外，芒果苷在 40、80 和 120 mg/kg 治疗时降低了 PBMC 和腹膜腔巨噬细胞的吞噬作用。然而，在 40 和 120 mg/kg 剂量下，它还增加了 NK 细胞活性。三个检查剂量对 T 细胞和 B 细胞增殖没有影响。在存活率研究中，芒果苷在 40 和 120 mg/kg 治疗的白血病小鼠体内显着提高存活率。