Biochar is a by-product from the pyrolysis of biomass and has a great potential in soil amendment due to its carbon and nutrient-rich properties. The aim of this study was to investigate the impact of increasing amounts (0, 0.01, 0.1, 0.2, 0.5 and 1.0%) of two types of biochar (so-called enhanced and non-enhanced) to soil on the biodegradation of ¹⁴C-phenanthrene. Enhanced biochar contains inoculants which are designed to potentially stimulate microbial activity and promote biological function in soil. After 100 d of incubation, the addition of 0.5% and 1% enhanced (EbioC) and non-enhanced biochars (NEbioC) led to longer lag phases, reduced rates and extents of ¹⁴C-phenanthrene in amended soil. However, in soils amended with 0.01%, 0.1% and 0.2% amendments, extents of mineralisation of ¹⁴C-phenanthrene increased and were found to be higher in the EBioC — as compared to the NEbioC-amended soils. Increasing soil-phenanthrene contact time also increased ¹⁴C-phenanthrene mineralisation in soil which had received smaller amounts of EBioC. Application of both EbioC and NEbioC also enriched the soil microbial populations during the incubation. However, it was found that phenanthrene-degrading microbial populations declined as soil contact time increased; this was particularly true for soils receiving larger amounts due to reduction in the mobile/bioaccessible fraction of the phenanthrene in soil. The findings revealed the importance of the type and amount of biochar that may be added to soil to stimulate or enhance organic contaminant biodegradation.

生物炭是生物质热解的副产物，由于其碳和养分丰富的特性，在土壤改良方面具有巨大潜力。这项研究的目的是研究两种类型的生物炭（所谓的增强型和非增强型）对土壤中¹⁴ C生物降解的增加量（0、0.01、0.1、0.2、0.5和1.0％）-菲。增强型生物炭含有设计成潜在刺激微生物活性并促进土壤生物学功能的孕育剂。孵育100天后，添加0.5％和1％增强的（EbioC）和非增强型生物炭（NEbioC）导致更长的迟滞期，降低的发生率和范围¹⁴改良土壤中的C-菲。但是，在用0.01％，0.1％和0.2％的改良剂改良的土壤中，与通过NEbioC改良的土壤相比，EBioC中¹⁴ C菲的矿化程度增加了，并且发现更高。增加土壤-菲接触时间也增加了¹⁴在已接受少量EBioC的土壤中C-菲的矿化作用。在孵化过程中，EbioC和NEbioC的应用也丰富了土壤微生物种群。然而，人们发现随着土壤接触时间的增加，降解菲的微生物数量减少了。对于由于土壤中菲的可移动/生物可及部分减少而接受大量土壤的情况尤其如此。该发现揭示了可添加到土壤中以刺激或增强有机污染物生物降解的生物炭的类型和数量的重要性。