Objective

History of the utilization of various parts of Camellia sinensis such as seed oil in different folklore medicaments and plants leaves for preparing green tea is very old. Pharmacokinetics (PK) plays a key role in the drug discovery stage by channelizing the medicinal chemist in the optimization process of chemical sequences and lends a hand to pharmacologists to invent in vivo studies. The objectives of the present study were to elaborate quantitative determination of phytochemicals and nutritional assessment of C. sinensis especially on the composition of seed oil of the local C. sinensis plant and to evaluate the pharmacokinetics of leaves extract in healthy volunteers.

Method

Determination of secondary metabolites and nutritional assessment of C. sinensis were carried out. The composition of seed oil was elaborated with the help of GC–MS analysis for its potential significance and evidence-based household uses as folklore but also for industrial applications. Pharmacokinetics of water extract of C. sinensis leaves was compared in 9 healthy human volunteers and divided into three groups (n = 3, in each) fasting, fed and fed with coke. Plasma concentrations were noted in each group and were quantified using a validated HPLC method.

Results

Phytochemical analysis and proximate analysis of C. sinensis suggests the presence of alkaloids, flavonoids, tannins, saponins sterols; and protein, lipids fiber and carbohydrate respectively. GC–MS of the C. sinensis seed oil shows the presence of linoleic acid 100%, palmitic acid 33.55%, stearic acid 21.96, caffeine 21.72 and vitamin E 14.49%. Pharmacokinetic studies suggest that in the fasting group, higher plasma concentration was found among all the volunteers.

Conclusion

The phytochemical analysis of green tea suggests that it is a good source of secondary metabolites full of important nutrients. GC–MS of seed oil reveals that seed of C. sinensis is a rich source of fatty acid, and can be used to fulfill the pharmaceutical need. Pharmacokinetic studies may help to develop the biomarkers for the green tea utilization and also would help to design the dose and dose frequency for achieving the significant absorption level. PK and PD also help to determine its plasma concentration and therapeutic efficacy.

中文翻译：

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茶油GC-MS分析的测定和茶树叶片提取物的药代动力学评估。

目的

利用茶花的各个部位（例如在不同的民间传说药物中使用种子油和用于制备绿茶的植物叶片）的历史非常悠久。药代动力学（PK）通过在化学序列的优化过程中引导药物化学家在药物发现阶段发挥关键作用，并帮助药理学家进行体内研究。本研究的目的是详细阐述中华绒螯蟹的植物化学成分的定量测定和营养评估，特别是当地中华绒螯蟹植物的种子油成分，并评估健康志愿者叶片提取物的药代动力学。

方法

进行了中华绒螯蟹次生代谢产物的测定和营养评估。借助GC-MS分析详细说明了种子油的成分，因为它具有潜在的意义，并且可以用作民俗和工业应用的循证家庭用途。在9名健康人类志愿者中比较了中华绒螯蟹叶片水提取物的药代动力学，并将其分为三组（每组n = 3）禁食，喂食和喂食可乐。记录每组的血浆浓度，并使用经过验证的HPLC方法进行定量。

结果

中华绒螯蟹的植物化学分析和近期分析表明，存在生物碱，类黄酮，单宁，皂苷固醇；分别是蛋白质，脂质纤维和碳水化合物。中华绒螯蟹种子油的GC-MS结果表明，存在100％的亚油酸，33.55％的棕榈酸，21.96％的硬脂酸，21.72％的咖啡因和14.49％的维生素E。药代动力学研究表明，在空腹组中，所有志愿者的血浆浓度较高。

结论

绿茶的植物化学分析表明，绿茶是富含重要营养素的次生代谢产物的良好来源。种子油的GC–MS分析表明，中华茶树种子富含脂肪酸，可用于满足制药需求。药代动力学研究可能有助于开发利用绿茶的生物标志物，也有助于设计剂量和剂量频率以达到显着的吸收水平。PK和PD也有助于确定其血浆浓度和治疗效果。