Abstract The goal of this study was to check the repeatability of changes of elemental composition of tissues introduced by formalin fixation followed by paraffin embedding (FFPE) – standard processing protocol used in histopathology. To achieve that goal two series of ten samples of quasi-homogeneous swine liver samples – lyophilised and FFPE processed – were compared in terms of statistical dispersion of their elemental concentrations. Total Reflection X-Ray Fluorescence (TXRF) was used for quantification of K, Ca, Mn, Fe, Cu, Zn, Se Br, Rb and Sr in samples after their acid digestion. It has been shown that relative standard deviation introduced by the statistical dispersion of the FFPE processing typically does not exceed 30% which may open up the possibility of analysis of large population of archival tissues.

中文翻译：

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对大量福尔马林固定石蜡包埋组织样品进行元素分析的可行性研究——初步结果

摘要 本研究的目的是检查由福尔马林固定后石蜡包埋 (FFPE) 引入的组织元素组成变化的可重复性 - 组织病理学中使用的标准处理协议。为了实现这一目标，我们对两个系列的 10 个准均质猪肝样品——冻干的和 FFPE 处理的——进行了元素浓度​​统计分散的比较。使用全反射 X 射线荧光 (TXRF) 对酸消解后的样品中的 K、Ca、Mn、Fe、Cu、Zn、Se Br、Rb 和 Sr 进行定量。已经表明，由 FFPE 处理的统计分散引入的相对标准偏差通常不超过 30%，这可能会开启对大量档案组织进行分析的可能性。