The present study aimed to investigate the effect of different Agrobacterium rhizogenes on the induction of hairy root of Cucumis anguria and determine its total phenolic, flavonoids contents, antibacterial and antioxidant activity. In this investigation A. rhizogenes strains such as, 15834, 13333, A4, R1200, R1000, LBA9402, R1301 and R1601 are all investigated, were developing hairy root conception in cotyledon and leaf tissue explants. Polymerase chain response (PCR) and the converse transcription-PCR are transgenic clones of hairy roots has been utilized rolA and rolB particular primers. In the middle of the different attention of better regulators the extreme transformation frequency was achieved in (IBA + NAA) cotyledon explant. Transgenic hairy roots increase in MS liquid medium added to with IBA + NAA (2.46 + 1.07) displayed the maximum accumulation of biomass 0.68 g/l dry weight (DW) and 6.52 ± 0.49 g/l fresh weight (FW) were obtained at the 21 days of cotyledon explant. The flavonoid and total phenolic contents were estimated using aluminium chloride method and Folin-Ciocalteu method. The amount of phenolic compounds in Cucumis anguria L non transformed root (124.46 ± 6.13 mg GA/g) was lower than that in the methanol extracts of Cucumis anguria L. hairy roots (160.38 ± 5.0 mg GA/g), being was Cucumis anguria L non transformed root lower (42.93 ± 1.58 mg rutin/g) than that in the concentration of total flavonoids in Cucumis anguria L. hairy root (16.26 ± 1.84 mg rutin/g). Additionally, transgenic hairy roots professionally produced various phenolic and flavonoid composites. The total antimicrobial activity, phenolics, flavonoids content and antioxidant were more in the hairy roots related to non-transformed roots. In our discovery, the A. rhizogenes R1000 is promising candidate for hairy root initiation of C. anguria from cotyledone explants were realized large number of hairy roots compared with leaf explants. The antioxidant potential of methanol extracts of flavonoid and phenolic compounds from the hairy roots have great potential to treat various diseases.

本研究旨在探讨不同发根土壤杆菌对黄瓜Cucumis anguria毛状根的诱导作用，并测定其总酚，类黄酮含量，抗菌和抗氧化活性。在这项研究中，发根农杆菌菌株，例如15834、13333，A4，R1200，R1000，LBA9402，R1301和R1601均已进行了研究，它们在子叶和叶片组织外植体中正在形成毛状根。聚合酶链反应（PCR）和逆转录-PCR是毛状根的转基因克隆，已被rol A和rolB特殊引物。在更好的监管者的不同关注中，（IBA + NAA）子叶外植体获得了极高的转化频率。添加IBA + NAA（2.46 + 1.07）的MS液体培养基中转基因毛状根的增加显示最大生物量积累为0.68 g / l干重（DW）和6.52±0.49 g / l新鲜重（FW）。子叶外植21天。使用氯化铝法和Folin-Ciocalteu法估算黄酮和总酚含量。黄瓜（Cucumis anguria L）未转化根中的酚类化合物含量（124.46±6.13 mg GA / g）低于黄瓜（Cucumis anguria L.）甲醇提取物中的毛状根（160.38±5.0 mg GA / g），即黄瓜（Cucumis anguria）L未转化根比黄瓜角状毛状根中总黄酮含量低（42.93±1.58 mg芦丁/g）（16.26±1.84 mg芦丁/ g）。此外，转基因毛根专业生产了各种酚醛和类黄酮复合材料。与未转化的根相关的毛状根中的总抗菌活性，酚类，类黄酮含量和抗氧化剂含量更高。在我们的发现中，发根农杆菌R1000是从子叶外植体毛an根毛状根引发的有希望的候选者，与叶外植体相比，已认识到大量毛状根。毛发根中黄酮类和酚类化合物的甲醇提取物的抗氧化潜力具有治疗各种疾病的巨大潜力。